Alginate Oligomers Inhibit Growth of Bacteria Causing Bovine Mastitis and Potentiate the Activity of Antibiotics Commonly Used For Treatment of the DiseaseAnne Tøndervik1*, Geir Klinkenberg1, Randi Aune1, Philip D Rye2 and Håvard Sletta1
- *Corresponding Author:
- Anne Tøndervik
SINTEF Materials and Chemistry, Department of Biotechnology and Nanomedicine
Sem Sælands veg 2A, 7034 Trondheim, Norway
Tel: +47 45054343
E-mail: [email protected]
Received date: August 01, 2017; Accepted date: August 24, 2017; Published date: September 01, 2017
Citation: Tøndervik A, Klinkenberg G, Aune R, Rye PD, Sletta H (2017) Alginate Oligomers Inhibit Growth of Bacteria Causing Bovine Mastitis and Potentiate the Activity of Antibiotics Commonly Used For Treatment of the Disease. J Adv Dairy Res 5:186. doi: 10.4172/2329-888X.1000186
Copyright: © 2017 Tøndervik A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Alginate oligomers have been shown to disrupt microbial biofilms and reduce minimum inhibitory concentrations (MIC) for many clinically relevant antibiotics to a range of human pathogens. The antibiofilm and potentiating effects of alginate oligomers has previously been focussed on bacterial and fungal strains that play significant roles in chronic human infections. This study on the effects of an alginate oligomer (OligoG CF-5/20) on bacteria that cause bovine mastitis is the first report that investigates a significant disease in animal health. The effect of OligoG CF-5/20 on the inhibitory concentrations of ampicillin, erythromycin, cephalothin and lincomycin to a test panel of nine bacterial strains associated with bovine mastitis were investigated. Two different media were used for cultivation, the standard Mueller-Hinton normally used for bacterial MIC determination, and a skimmed milk medium to mimic the native growth conditions for mastitis bacteria. OligoG CF-5/20 was shown to inhibit growth of all strains tested, and demonstrated a 2 to 8 fold reduction in MICs for erythromycin, cephalothin and lincomycin. The data highlights a potential role for alginate oligomers in potentiating the efficacy of antibiotics, and thereby potentially reducing antibiotic use, in the treatment of mastitis in dairy cattle.