Alkali Pre-Treatment Optimization of Sardine Scales
Response surface methodology (RSM) was adopted for pre-treating optimization. Concentration of NaOH (X1) and treatment time (X2) were chosen for independent variables. Dependent variables were protein content (Y1) and hydroxyproline content (Y2). Optimal conditions were X1=0,5% and X2=4h , and predicted values of multiple response optimal conditions were Y1=0,32 mg/l and Y2=2,67mg/l. That result was in agreement with the predicted value, which indicates that the model used was adequate for pre-treatment.