alexa Allelic Diversity of Polymorphic AMA-1 (Apical Membrane
ISSN: 2329-891X

Journal of Tropical Diseases & Public Health
Open Access

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Research Article

Allelic Diversity of Polymorphic AMA-1 (Apical Membrane Antigen 1) Vaccine Candidate Antigen of Plasmodium falciparum in Two Population of Imported and Indigenous Cases in South-East of Iran using Nested-PCR and RFLP

Adel Ebrahimzadeh*, Abdolaziz Gharaei and Khadije Saryazdi

Department of Medical Parasitology and Mycology, Zahedan University of Medical Sciences, Zahedan, Iran and membership in Infectious Diseases and Tropical Medicine Research Center ,Zahedan University of Medical Sciences, Zahedan, Iran

*Corresponding Author:
Ebrahimzadeh A, PhD
Associated Professor
Department of Medical Parasitology and Mycology
Zahedan University of Medical Sciences, Zahedan
Iran and membership in Infectious Diseases
and Tropical Medicine Research Center Zahedan
University of Medical Sciences, Zahedan, Iran
Tel: +98-915-5491303
Fax: +985412416670
E-mail: [email protected]; [email protected]

Received Date: March 16, 2014; Accepted Date: September 27, 2014; Published Date: October 03, 2014

Citation: Ebrahimzadeh A, Gharaei A, Saryazdi K (2014) Allelic Diversity of Polymorphic AMA-1 (Apical Membrane Antigen 1) Vaccine Candidate Antigen of Plasmodium falciparum in Two Population of Imported and Indigenous Cases in South-East of Iran using Nested-PCR and RFLP. J Trop Dis 2:149. doi:10.4172/2329-891X.1000149

Copyright: © 2014 Ebrahimzadeh A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

The Plasmodium falciparum Apical Membrane Antigen 1 (AMA1) is a leading Malaria Vaccine Candidate Antigen. Antigenic variation is one of the main obstacles in the development of a universal effective malaria vaccine. Antibodies against AMA1 have been shown to block parasite invasion of human erythrocytes. Therefore, detailed studies on the molecular polymorphism PFAMA1 a Geographic area before each experiment or design the vaccine is administered. This study was designed to determine the distribution of AMA1 allele class of Plasmodium falciparum in two population of imported cases and indigenous cases in South-East of Iran .We used the Nested PCR and RFLPs methods with specific primers and restriction enzymes, which improves the three AMA1 allele class(K1-3D7-HB3).Overall the 94 confirmed P. falciparum samples obtained from four different districts, in two population of imported cases(46) and indigenous cases(48) in the south East of Iran. There are three classes of allelic AMA1 were compared in two populations of Indigenous and Imported, 3D7 in Indigenous Population was the higher prevalence than Imported Population. Considering on these results, 3D7 alleles suitable candidates for malaria vaccine design can be. The data reported here will be valuable for the development of AMA1 based malaria vaccine.

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