alexa Alternative Silver Stain Detection protocol for the GenePrint® STR System Applied to Mestizo Population From Jalisco (West of Mexico) | OMICS International | Abstract
ISSN: 2157-7145

Journal of Forensic Research
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Alternative Silver Stain Detection protocol for the GenePrint® STR System Applied to Mestizo Population From Jalisco (West of Mexico)

Rangel-Villalobos H1*, Salazar-Flores J1, Muñoz-Valle JF2, Torres-Jasso JH1 and Páez-Riberos LA3

1Instituto de Investigación en Genética Molecular (CUCiénega-UdeG) , Departamento de Ciencias Médicas y de la Vida, Centro Universitario de la Ciénega, Universidad de Guadalajara (CUCI-UdeG) , Ocotlán, Jalisco, México.

2Instituto de Investigación en Reumatología y del Sistema Músculo Esquelético (CUCS-UdeG), Departamento de Genómica y Biología Molecular, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, México

3Departamento de Ciencias de la Tierra y de la Vida, Centro Universitario de los Lagos (CULagos-UdeG), Departamento de la Vida y de la Tierra, Universidad de Guadalajara, Lagos de Moreno, Jalisco, México

*Corresponding Author:
Héctor Rangel Villalobos
Instituto de Investigación en Genética Molecular
Centro Universitario de la Ciénega (CUCI-UdeG)
Av. Universidad # 1115, Col Paso Blanco,CP. 47810
Ocotlán, Jal., MEXICO
Tel: +52-(392) 92 59400 ext. 8363
FAX: +52-(392) 92 57112
E-mail: [email protected]

Received date: November 05, 2010; Accepted date: November 29, 2010; Published date: December 02, 2010

Citation: Rangel-Villalobos H, Salazar-Flores J, Muñoz-Valle JF, Torres-Jasso JH, Páez-Riberos LA (2010) Alternative Silver Stain Detection protocol for the GenePrint® STR System Applied to Mestizo Population From Jalisco (West of Mexico). J Forensic Res 1:105. doi:10.4172/2157-7145.1000105

Copyright: © 2010 Rangel-Villalobos H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A population sample of 163 unrelated individuals from the Mexican state of Jalisco (West of Mexico) was PCRtyped with the Geneprint® STR System including nine autosomal STRs (CSF1PO, TPOX, TH01, F13A01, FESFPS, vWA, D16S539, D7S820 and D13S317). For this purpose, we implemented an alternative protocol for STR silver stain detection, which provides some economical and technical benefits: 1) make easy the gel handling; 2) simplify the process avoiding the use of some –toxic– reagents; 3) require lesser timer and made-in-home solutions; 4) the original gels can be dried and stored for re-analysis, if necessary. The allele frequencies estimated were similar to a previous report from the same Mexican population, which allowed pooling STR genotype data for upgrade the forensic parameter estimates (n= 472). However, this is the first –and unique– population study in Mexico for F13A01 and FESFPS; thus, the sample size did not increased for these loci (n= 163). In the studied population, genotype distribution by locus and two-loci combination was in agreement with Hardy-Weinberg expectations for all nine STRs. For the STR system, the power of discrimination (PD) and exclusion (PE) were >99.999% and 99.9%, respectively.

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7