alexa Amifostine Protects the White Cell Line Against Genotoxic Damage in Patients Undergoing Pelvic Radiotherapy | OMICS International | Abstract
ISSN: 2161-0495

Journal of Clinical Toxicology
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Amifostine Protects the White Cell Line Against Genotoxic Damage in Patients Undergoing Pelvic Radiotherapy

Pouliliou Stamatia1, Papaiakovou Marina1, Kareli Dimitra2, Lialiaris Theodore2 and Koukourakis I Michael1*

1Department of Radiotherapy/Oncology, Democritus University of Thrace, Greece

2Department of Medical Biology and Cytogenetics, Genetics Laboratory, Democritus University of Thrace, Greece

*Corresponding Author:
Michael I. Koukourakis
Department of Radiotherapy/Oncology
Democritus University of Thrace, PO BOX 12
Alexandroupolis 68100, Greece
Tel: 30-25510-74622
Fax: 30-25510-30349
E-mail: [email protected]

Received date: July 10, 2012; Accepted date: August 27, 2012; Published date: August 30, 2012

Citation: Stamatia P, Marina P, Dimitra K, Theodore L, Koukourakis I Michael (2012) Amifostine Protects the White Cell Line Against Genotoxic Damage in Patients Undergoing Pelvic Radiotherapy. J Clinic Toxicol 2:139. doi: 10.4172/2161-0495.1000139

Copyright: © 2012 Stamatia P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

X-rays are widely used in the treatment of cancer. Nevertheless, radiation may induce damage to normal tissues, through free radical release and Deoxyribonucleic acid (DNA) damage. Amifostine is a broad spectrum cytoprotective agent with free radical scavenger and DNA repair activity that reduces toxicity during radiotherapy and chemotherapy. The aim of the present in vivo study was to investigate the cytoprotective efficacy of amifostine against chromosomal damage induced on peripheral blood lymphocytes in cancer patients under radiotherapy for pelvic tumors. The levels of genotoxicity, cytostaticity and cytotoxicity were quantitatively evaluated using the Sister Chromatid Exchange (SCE) methodology, the Proliferation Rate Index (PRI), the Mitotic Index (MI). PBMCs were collected at 0, 5 and 19 days from patients with pelvic tumors undergoing daily fractionated radiotherapy. Five patients received Radiotherapy (RT) alone (Group A) and 5 received RT supported with amifostine (1000 mg sc.) (Group B) before each RT fraction. A gradual increase of SCEs was noted in group A (p=0.03) and a rather stable score in group B by increasing the days of radiotherapy. At day 19 a significant increased SCE number characterized group A (p=0.005). The mitotic index was significantly reduced by increasing the days of radiotherapy in both groups A and B (p=0.02 and 0.01 respectively) and there was no difference between groups at the various time points. The proliferation rate index was not affected. Pelvic radiation induces a significant amount of SCEs in PBMCs and reduces the mitotic index, implying a potential leukemogenic and certainly cytotoxic activity. Amifostine daily administration although did not protect against cytotoxic damage it reduced the amount of SCEs suggestive of a protection against the stochastic effects of radiation.

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

bornova escort

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version