alexa Amount of Haemophilus influenzae Genomic DNA in Middle
ISSN: 2155-9597

Journal of Bacteriology & Parasitology
Open Access

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Research Article

Amount of Haemophilus influenzae Genomic DNA in Middle Ear Fluids of Pediatric Acute Otitis Media

Atsuko Masuno1#, Muneki Hotomi1*#, Akihisa Togawa1, Rinya Sugita2 and Noboru Yamanaka1

1Department of Otorhinolaryngology-Head and Neck Surgery, Wakayama Medical University, Wakayama, Japan

2Sugita ENT Clinic, Chiba, Japan

#Muneki Hotomi and Atsuko Masuno contribute equally in this study

*Corresponding Author:
Muneki Hotomi
Department of Otolaryngology-Head
and Neck Surgery, Wakayama Medical University
811-1, Kimiidera, Wakayama-shi
Wakayama, 641-8510, Japan
Tel: +81-73-441-0651
Fax: +81-73-446-3846
E-mail: [email protected]

Received Date: January 04, 2016 Accepted Date: January 25, 2016 Published Date: January 29, 2016

Citation: Masuno A, Hotomi M, Togawa A, Sugita R, Yamanaka N (2016) Amount of Haemophilus influenzae Genomic DNA in Middle Ear Fluids of Pediatric Acute Otitis Media . J Bacteriol Parasitol 7:259. doi: 10.4172/2155-9597.1000259

Copyright: © 2016 Masuno A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Background: Nontypeable Haemophilus influenzae (NTHi) is a leading causative pathogen responsible for acute otitis media (AOM).
Objective: In this study, we applied real-time PCR to quantify the amount of genomic NTHi DNA in MEEs and to evaluate the influence of the amount of bacteria in MEEs on the clinical outcome of AOM.
Methods: Thirty-two children with severe AOM were evaluated for the quantification of the amount of NTHi genomic DNA in middle ear effusions (MEEs) by real-time PCR.
Results: The improvement of tympanic membrane abnormalities at the second visits was significantly worse in cases with MEEs containing dense NTHi genomic DNA rather than in cases with MEEs containing sparse NTHi genomic DNA. The amount of NTHi DNA genome in MEEs were significantly higher in cases of which improvement ratio of tympanic membrane abnormalities at the second visit was less than 50% than in cases of which improvement ratio of tympanic membrane abnormalities above 50%.
Discussion: It is important to predict the clinical futures of AOM for appropriate treatments. The quantity of infected pathogens will be an important factor for poor clinical outcomes of AOM.
Conclusion: The current findings suggest that severity of tympanic membrane abnormalities evaluated scoring system proposed by Japanese AOM guideline reflect the amount of NTHi in MEEs and that predict the poor improvement of AOM.


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