alexa An Alternative Methodology for Determination of Cephamycin C from Fermentation Broth | OMICS International | Abstract
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
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Research Article

An Alternative Methodology for Determination of Cephamycin C from Fermentation Broth

Álvaro de Baptista Neto1, Liliane Maciel de Oliveira2, Carolina Bellão2, Alberto Colli Badino Jr2, Marlei Barboza2* and Carlos Osamu Hokka2

1Verdartis- Desenvolvimento Biotecnológico Ltda – ME, Brazil

2Departamento de Engenharia Química - Universidade Federal de São Carlos, Brazil

*Corresponding Author:
Marlei Barboza,
Programa de Pós-Graduação em Engenharia Química
Universidade, Federal de São Carlos
Via Washington Luís, km 235
SP-310, CP 676 CEP 13565-905
São Carlos, SP, Brazil
Tel: + 55 16 33518264
Fax: + 55 16 33518266
E-mail: [email protected]

Received date: May 07, 2012; Accepted date: July 11, 2012; Published date: July 15, 2012

Citation: de Baptista Neto Á, de Oliveira LM, Bellão C, Badino AC Jr, Barboza M, et al. (2012) An Alternative Methodology for Determination of Cephamycin C from Fermentation Broth. J Chromat Separation Techniq 3:130. doi:10.4172/2157-7064.1000130

Copyright: © 2012 de Baptista Neto Á, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Cephamycin C is a β-lactam antibiotic used as a raw material in several commercial antibiotics. The production and purification process of this antibiotic requires a fast, simple and accurate method to quantify it. In this paper, it was developed a high-performance liquid chromatography method to determine cephamycin C concentration, in order to offer an option for the traditional, but laborious, bioassay method normally employed. A method to obtain a calibration curve using the bioassay and cephalosporin C as standard was also proposed. The method showed more efficiency in determining cephamycin C than the bioassay, and it was simpler and faster to execute.

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