An Alternative Technique for Heart Lesion in the Rat: A Step to Fetal Heart Implantation for Cardiac Tissue Repair Running Title: A New Technique of Heart Lesion in Rat
- *Corresponding Author:
- Very Coulic
Laboratory of Experimental Medicine
Université Libre de Bruxelles
Centre Hospitalier Universitaire
Brugmann site Horta, Brussels, Belgium
E-mail: [email protected]
Received Date: July 24, 2015; Accepted Date: September 29, 2015; Published Date: October 04, 2015
Citation: Coulic V, Queron S, Stefanidis C, Delree P, Prez CD,et al. (2015) An Alternative Technique for Heart Lesion in the Rat: A Step to Fetal Heart Implantation for Cardiac Tissue Repair Running Title: A New Technique of Heart Lesion in Rat. J Transplant Technol Res 5: 149. doi: 10.4172/2161-0991.1000149
Copyright: © 2015 Coulic V, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Existing experimental models of cardiac lesion do not allow precise reproducible dimensions of injury with complete safeness of surrounding tissues and cannot answer the tremendous question for heart cell therapy: are the implanted cells unable to influence the heart healing process or do the conditions in the injured heart prevent their complete development and integration? 60 rats were used for elaborating an alternative technique of cardiac lesion. The anterior apical area of the heart was cauterized with a «Cautery high temperature fine tip» to create an injury of controllable, precise and reproducible dimensions (8×8 mm² surface, 1 mm depth). To evaluate functional and morphological characteristics of the lesion, electrocardiography, pulse oximetry, echocardiography and optic microscopy were performed at different times (from day 0 to 230) after the operation. After technical adjustment a 100% survival of the last 15 operated animals was obtained. A sub epicardium “infarction” was documented: ECG mirror ST-modifications in 2 leads, stable ejection fraction significant decrease, necrotic alterations and fibrosis of the lesion area, with surrounding myocardium preserved. The survival and the injury evolution suggest that the proposed technique could be used for studies concerning cardiac tissue repair.