alexa An Efficient Method for Extraction of Secreted Proteins of a Filamentous Fungus, Cryphonectria parasitica | OMICS International | Abstract
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

An Efficient Method for Extraction of Secreted Proteins of a Filamentous Fungus, Cryphonectria parasitica

Jinzi Wang, Fangzhen Wang, Jinjie Shang and Baoshan Chen*

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, China

*Corresponding Author:
Dr. Baoshan Chen
College of Life Science and Technology
Guangxi University, Nanning, 530004, PR China
Tel: +86 771 3239566
Fax: +86 771 3237873
E-mail: [email protected]

Received Date: May 09, 2011; Accepted Date: June 17, 2011; Published Date: June 20, 2011

Citation: Wang J, Wang F, Shang J, Chen B (2011) An Efficient Method for Extraction of Secreted Proteins of a Filamentous Fungus, Cryphonectria parasitica. J Proteomics Bioinform 4: 125-128. doi:10.4172/jpb.1000179

Copyright: © 2011 Wang J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Three different methods were evaluated for extraction of secreted proteins from a filamentous fungus, Cryphonectria parasitica, cultured in liquid medium. Protein samples prepared with each method were subjected to SDS-PAGE and two-dimensional gel electrophoresis (2-DE). It was found that ultrafiltration was not suitable for extraction of the secreted proteins. Protein samples prepared by chloroform treatment followed by ammonium sulfate precipitation were acceptable for 2-DE separation. The modified sevage method was the best to extract secreted proteins with highest yield and best quality. About 300 protein spots could be identified on a 2-DE gel using protein samples prepared with this method from 3 day-old culture. Successful isolation of high quality secreted proteins is the pre-request for the analysis of fungal secreted proteome. The new method may be applied to other filamentous fungi, particularly the pathogenic ones.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7