alexa Analysis of 258 Different Lesions of the Central Nervou
ISSN: 2376-0389

Journal of Multiple Sclerosis
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Research Article

Analysis of 258 Different Lesions of the Central Nervous System for Real Time Histopathological Diagnosis Using Confocal Laser Endomicroscopy

Samira Daali1*, Mehreen Javed1, Ann-Kristin Altekoester1, Maximilian Linxweiler2, Richard Bostelmann3, Juergen Schlegel4, Alhadi Igressa5#, Patra Charalampaki5#

1Department of Neurosurgery, Hospital Merheim, Cologne Medical Center, Germany

2Department of Otorhinolaryngology, Saarland University Medical Center, Saarland University, Germany

3Department of Neurosurgery, Heinrich Heine University Düsseldorf, Düsseldorf, Germany

4Department of Neuropathology, Technical University Munich, Munich, Germany

5Department of Neurosurgery, Hospital Merheim, Cologne Medical Center, Germany

#contributed equally

*Corresponding Author:
Mrs. Samira Daali
Department of Neurosurgery, Cologne Medical Center
Ostmerheimerstr. 200, 51103 Cologne, Germany
Tel.: +49 1729550615
Fax: +49-221-8907-13373
E-mail: [email protected]

Received date: February 19, 2016; Accepted date: March 31, 2016; Published date: April 07, 2016

Citation: Samira D, Mehreen J, Ann-Kristin A, Alhadi I, Maximilian L, et al. (2016) Analysis of 258 Different Lesions of the Central Nervous System for Real Time Histopathological Diagnosis Using Confocal Laser Endomicroscopy. J Mult Scler (Foster City) 3:169. doi:10.4172/2376-0389.1000169

Copyright: © 2016 Samira D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.





The safe removal of central nervous system tumors in or near eloquent tissue remains extremely challenging due to its complex anatomy. The injury of eloquent tissue can have serious consequences for both mental and physical health while an insufficiently resected tumor bears a high risk of tumor recurrence decreasing the patient’s life quality and quantity. The novel method, confocal laser endomicroscopy (CLE), promises safe tumor resection as it permits intraoperative histological imaging of tissues in real-time. Thus, aim of the current study was to evaluate CLE for a fast diagnosis of brain and spinal cord lesions in neurosurgery.

Methods: CLE was used for an ex vivo assessment of tumor samples from 258 diverse central nervous system lesions. Additionally, traditional histology was performed on the same examined tissue as the gold standard. Nonneoplastic brain tissue served as a control.

Results: The examination of brain and spinal biopsies using CLE allowed the identification of healthy tissue, primary brain and spinal tumors, metastases, abscesses and vascular malformations with a high accuracy of 88.64%. Confocal imaging provided precise cellular and sub-cellular details such as psammoma bodies in meningiomas, perivascular pseudorosettes in ependymomas, microvascular proliferation in glioblastomas and mitotic activity in highgrade tumors.

Conclusion: CLE is a promising method for distinguishing tumor from the surrounding healthy tissue, as well as for the immediate diagnosis of biopsies. Our studies have the potential to establish a faster preliminary diagnosis in an ongoing surgery as compared to current available methods. Moreover, the presented characteristic cellular and subcellular confocal features of examined tumors and non-neoplastic tissues could be used to guide future tumor surgeries to enable a more precise and safe resection.


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