Analysis of Cytokines in Presumed Acute Infectious Endophthalmitis Following Cataract Extraction
|Paulo Escarião1,2*, Alessandra Goncalves Commodaro3, Tiago Arantes2, Célia M. M. B. de Castro4, Maria de Fátima A. Diniz4 and Carlos Teixeira Brandt1|
|1 Department of Surgery, Federal University of Pernambuco, Brazil|
|2 Altino’s Ventura Foundation, Recife, Pernambuco, Brazil|
|3 Department of Ophthalmology, Federal University of Sao Paulo, Sao Paulo, Brazil|
|4 Laboratory of Immunopathology Keiso Asami (LIKA), Federal University of Pernambuco, Brazil|
|Corresponding Author :||Paulo Escarião
Universidade Federal de Pernambuco – UFPE
Av. Prof. Moraes Rego, 1235 - Cidade Universitária
Recife - PE CEP 50670-901, Brazil
E-mail: [email protected]
|Received: March 05, 2014; Accepted: April 28, 2014; Published: April 30, 2014|
|Citation: Escarião P, Commodaro AG, Arantes T, de Castro CMMB, Diniz MdeFA, et al. (2014) Analysis of Cytokines in Presumed Acute Infectious Endophthalmitis Following Cataract Extraction. J Clin Exp Ophthalmol 5:335. doi:10.4172/2155-9570.1000335|
|Copyright: © 2014 Escarião P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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Objective: To analyze the involvement of cytokines in acute infectious endophthalmitis following cataract extraction surgery.
Methods: Vitreous humor samples were collected from 18 patients. The multiplex technique was used to measure IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17, basic FGF, eotaxin, GCSF, GM-CSF, IFN-γ, MCP-1, MIP-1a, MIP-1b, PDGF-BB, RANTES, TNF-α and VEGF for comparison with levels in 39 patients undergoing vitrectomy for a non-infectious disease (control).
Results: IL-1ra, IL-2, IL-4, IL-6, IL-8, IL-9, IL-10, IL-15, IL-17, eotaxin, bFGF, G-CSF, GM-CSF, IFN-γ, MCP-1, MIP-1a, MIP-1b, PDGF-BB, RANTES and TNF-α levels were significantly higher (p <0.05) in eyes with endophthalmitis. There were no significant differences between groups for IL-5, IL-12 (p70), IL-13 and VEGF levels. IL-7 levels were higher in the control group (p=0.0229). Considering the different diagnosis in the control group, only IL-5 was not significantly different between groups (p=0.1764). There was a positive correlation between TNF-α and the initial visual acuity (r=0.59, p=0.0096). There was no correlation with the final visual acuity for the cytokines measured. The concentration of IL-8 was higher in patients with endophthalmitis and positive cultures (p=0.0250).
Conclusions: Infectious endophthalmitis following cataract extraction stimulates the production of cytokines. Levels of TNF-α may be related to the severity of the initial inflammatory processes. IL-8 levels are correlated with positive bacterial cultures, suggesting that IL-8 could be used as a biomarker for bacterial infection.