alexa Analysis of Fungal Diversity in Ready-to-Eat Pizza and Effectiveness of Pulsed Ultraviolet-Light Treatment for Inactivation of Mold on Agar Surface | OMICS International
ISSN: 2155-9821

Journal of Bioprocessing & Biotechniques
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Analysis of Fungal Diversity in Ready-to-Eat Pizza and Effectiveness of Pulsed Ultraviolet-Light Treatment for Inactivation of Mold on Agar Surface

Tina M Beigi1, Michael O Ngadi1*, Devin B Holman2 and Martin R Chénier2,3
1Department of Bio resource Engineering, McGill University, Ste-Anne-de-Bellevue, QC, Canada
2Department of Animal Science, McGill University, Ste-Anne-de-Bellevue, QC, Canada
3Department of Food Science and Agricultural Chemistry, McGill University, Ste-Anne-de-Bellevue, QC, Canada
Corresponding Author : Michael O Ngadi
Department of Bioresource Engineering
McGill University, 21, 111 Lakeshore Road
Ste-Anne-de-Bellevue
Quebec, Canada, H9X 3V9
Tel: +1-514-398-7923
Fax: +1-514-398-7990
E-mail: [email protected]
Received April 20, 2015; Accepted May 18, 2015; Published May 22, 2015
Citation: Beigi TM, Ngadi MO, Holman DB, Chénier MR (2015) Analysis of Fungal Diversity in Ready-to-Eat Pizza and Effectiveness of Pulsed Ultraviolet-Light Treatment for Inactivation of Mold on Agar Surface. J Bioprocess Biotech 5:228 doi: 10.4172/2215-9821.1000228
Copyright: © 2015 Beigi TM et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Fungal contamination is a significant issue in the food production industry; therefore, identification and characterization of food spoilage fungi would allow for early intervention to limit the amount of fungal contamination, particularly in cereal-based industries. In the present study, culture-dependent and culture-independent methods were applied to study the microbiota of ready-to-eat pizza. The study was pursued by evaluating the effectiveness of a broad-spectrum pulsed ultraviolet light for the decontamination of Penicillium roqueforti (a dominant spoilage mold in bakery products) on the surface of solid agar as a representative of a flat food surface. The average population of mesophilic aerobic bacteria (MAB), mesophilic anaerobic bacteria (MANB), lactic acid bacteria (LAB), molds and yeasts (M+Y) on naturally spoiled pre-cooked pizza were 6.7 ± 0.5, less than 2.3, 2.8 ± 0.6 and 5.4 ± 0.4 log10 CFU g-1, respectively. Cloning and sequencing identified at least 5 genera and species of fungi (Penicillium spp., Saccharomyces spp., Rhodotorula mucilaginosa, Monascus fuliginosus, Galactomyces geotrichum) from 2 phyla (Ascomycota and Basidiomycota). Pulsed light process parameters evaluated were treatment time (1, 3, 5, 7 and 10 min) and voltage input (500, 750 and 1,000 V) at 5 cm distance from the pulsed UV-light. An increase of the input voltage of the lamps and of the duration of the treatment resulted in a higher inactivation of P. roqueforti. The population of P. roqueforti was reduced after 10 min of exposure to pulsed light by 3.74, 5.36 and 6.14 log10 CFU ml-1 at 500, 750 and 1,000 V, respectively. The inactivation kinetics was best described by the Weibull model (2 parameters) with the smallest root mean squared error (RMSE) and R2 ≥ 0.92. The results of this work show that pulsed light is a promising technique for fungi elimination or decontamination in the bakery industry.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version