Analysis of Synapsin I and II mRNAs and Proteins During Murine Development
Department of Biology, University of Puerto Rico at Cayey, Puerto Rico
- *Corresponding Author:
- Michael RR
Department of Biology
University of Puerto Rico at Cayey
E-mail: [email protected]
Received date: June 12, 2017; Accepted date: June 23, 2017; Published date: June 30, 2017
Citation: Michael RR (2017) Analysis of Synapsin I and II mRNAs and Proteins During Murine Development. Mol Biol 6:192. doi:10.4172/2168-9547.1000192
Copyright: © 2017 Michael RR. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The synapsins are a family of neuronal phosphoproteins that have been previously shown to play an important regulatory role in the release of neurotransmitter from the presynaptic terminal and in the process of synaptogenesis. The mechanisms that regulate the formation of synaptic terminals are a central process in determining the specificity of synaptogenesis and the development of the nervous system. Proteins involved in neurotransmitter release and the control of this release process (specifically synaptic vesicle proteins) have been implicated as being important for synaptogenesis. To determine whether synapsin is expressed at times during mouse development when synaptogenic activity is high, we examined the time course of synapsin I and II mRNA and protein expression in embryonic and postnatal mice using Northern blot and Western blot analyses. Quantification of these blot analyses demonstrated that synapsin RNA and protein can be detected as early as 13.5 days of mouse embryogenesis and that expression of five of the six isoforms of synapsin increase throughout embryonic and postnataldevelopment reaching characteristic high levels by adulthood. This early expression pattern suggests an important role for the synapsins in the development of the mammalian nervous system.