Analysis of the APO B R3500Q Mutation and APOE Polymorphism in Taif Saudi Population using Polymerase Chain Reaction- Reveres Hybridization Technique
Nabil Saied Awad* and Adel El-Sayed El-Tarras
College of Medicine, Biotechnology and Genetic Engineering Unit, Taif University, Taif, Kingdom Saudi Arabia
- *Corresponding Author:
- Nabil Saied Awad
College of Medicine
Biotechnology and Genetic Engineering Unit
Taif University, Taif, Kingdom Saudi Arabia
E-mail: [email protected]
Received Date: May 05, 2011; Accepted Date: June 20, 2011; Published Date: June 28, 2011
Citation: Awad NS, El-Tarras AE (2011) Analysis of the APO B R3500Q Mutation and APOE Polymorphism in Taif Saudi Population using Polymerase Chain Reaction- Reveres Hybridization Technique. J Mol Biomark Diagn 2:109. doi:10.4172/2155-9929.1000109
Copyright: © 2011 Awad NS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
This study describes the use of Cardio Vascular Disease (CVD) Strip Assay which based on Polymerase Chain Reaction Reverse Hybridization Technique to study the prevalence of Apo B R3500Q mutation and Apo E genotypes in Taif city Saudi Arabian population. Among 200 unrelated healthy subjects residing in Taif city 5,600 feet above sea level complete absence of Apo B R3500Q mutation was observed. Among six different Apo E genotypes, five genotypes were detected (E3/E3, E4/E4, E2/E3, E2/E4 and E3/E4) with prevalence% (63, 2.5, 8.5,1 and 25) respectively. The allelic frequencies of Apo E alleles were 0.79 for E3, 0.15 for E4 and 0.048 for E2.Obtained data concluded that, this is the first report about Apo B R3500Q mutation in Saudi Arabia and the absence of it confirmed that this mutation has not reached Saudi Arabia from central Europe which is the common origin of this mutation. Among Apo E genotypes, only E2/E2 genotype was absent while another E2/E3 and E2/E4 genotypes were detected in Taif Saudi population.