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Analysis of the Sequence of ITS1-5.8S-ITS2 Regions of the Three Species of <em>Fructus Evodiae</em> in Guizhou Province of China and Identification of Main Ingredients of Their Medicinal Chemistry | Abstract
ISSN: 0974-7230

Journal of Computer Science & Systems Biology
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Research Article

Analysis of the Sequence of ITS1-5.8S-ITS2 Regions of the Three Species of Fructus Evodiae in Guizhou Province of China and Identification of Main Ingredients of Their Medicinal Chemistry

Liu-Zhongyu1, Liu-Yang2, Liu-Shouhan1, Ding-Xianping1, Yang-Yi1* and Huang-Hai2*

1College of Life Science, Sichuan University, China. 610041

2Department of Clinical Biochemistry, Guiyang Medical College China. 550004

*Corresponding Author:
Dr. Yang-Yi
College of Life Science
Sichuan University, China. 610041
E-mail : [email protected]

Dr. Huang-Hai
Department of Clinical Biochemistry
Guiyang Medical College China. 550004
E-mail : [email protected]

Received date: May 07, 2009; Accepted date: June 23, 2009; Published date: June 24, 2009

Citation: Liu Z, Liu Y, Liu S, Ding X, Yang Y et al. (2009) Analysis of the Sequence of ITS1-5.8S-ITS2 Regions of the Three Species of Fructus Evodiae in Guizhou Province of China and Identification of Main Ingredients of Their Medicinal Chemistry. J Comput Sci Syst Biol 2:200-207. doi:10.4172/jcsb.1000032

Copyright: © 2009 Liu Z, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A total of fourteen specimens of three Fructus Evodiae species were collected from Zunyi and Tongren of Guizhou Province in China. The internal transcribed spacer (ITS) region (ITS1, ITS2 and 5.8S rDNA) of the nuclear ribosomal DNA (nrDNA) of the fourteen species were amplified and sequenced and another two sequences of Evodia rutaecarpa (JUSS.) BENTH species were obtained from Hunan Academy of Traditional Chinese Medicine, Changsha, Hunan Province of China. In addition to, the main ingredients of their medicinal chemistry of all specimens were identified by high performance liquid chromatography (HPLC). The three different morphological Fructus Evodiae species could not be distinctly classified by phylogenetic analysis of ITS combining the main ingredients of medicinal chemistry. This conclusion is inconsistent with early research results.

Keywords

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