Analytical Determination of Buspirone Hydrochloride in Pure Solutions, Pharmaceutical Preparation and Urine Samples
- *Corresponding Author:
- Mostafa M
Forensic Chemistry lab
Forensic medicine authority
Ministry of Justice, Egypt
E-mail: [email protected]
Received Date: November 11, 2014; Accepted Date: January 30, 2015; Published Date: February 06, 2015
Citation: Abdel-Ghani NT, Rizk MS, Mostafa M2 (2015) Analytical Determination of Buspirone Hydrochloride in Pure Solutions, Pharmaceutical Preparation and Urine Samples. Pharm Anal Acta 6:334. doi: 10.4172/2153-2435.1000334
Copyright: ©2015 Abdel-Ghani NT, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Simple, rapid, sensitive, precise and accurate spectrophotometeric methods for the determination of Buspirone hydrochloride (Bu-HCl) in bulk samples, dosage form and in spiked urine samples were investigated. The methods are based on the formation of a yellow colored ion-associates due to the interaction between the examined drug with Picric acid (PA), Bromothymol blue (BTB), Alizarin red (AR) and Bactophenol red (BPR) reagents. A buffer solution had been used and the extraction was carried out using organic solvent, the ion associates exhibit absorption maxima at 410, 410, 430 and 423 nm of (Bu-HCl) with PA, BBT, AR and BPR respectively. (Bu-HCl) could be determined up to 42.5, 29.5, 73.8 and 105.5 μg mL-1, using PA, BBT, AR and BPR respectively. The optimum reaction conditions for quantitative analysis were investigated. In addition, the molar absorptivity and Sandell sensitivity were determined for the investigated drug. The correlation coefficient was ≥ 0.995 (n=6) with a relative standard deviation (RSD) ≤ 2.66 for five selected concentrations of the reagents. Therefore the concentration of Bu-HCl in its pharmaceutical formulations and spiked urine samples had been determined successfully.