Analyzing Intracellular flux Distributions of Thermoanaerobacter sp.X514 under Various Substrate Conditions by Gibbs Sampling
Jing Yang1, Xiaofang Ling1, Tian Xie1, Lishan Yao1 and Visakan Kadirkamanathan2*
1Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, P.R.China
2Department of Automatic Control & Systems Engineering, University of Sheffield, Sheffield, South Yorkshire, United Kingdom
- *Corresponding Author:
- Visakan Kadirkamanathan
Department of Automatic Control & Systems Engineering
University of Sheffield, Sheffield
South Yorkshire, United Kingdom
E-mail: [email protected]
Received date: March 27, 2012; Accepted date: May 03, 2012; Published date: May 07, 2012
Citation: Yang J, Ling X, Xie T, Yao L, Kadirkamanathan V (2012) Analyzing Intracellular flux Distributions of Thermoanaerobacter sp. X514 under Various Substrate Conditions by Gibbs Sampling. J Biomet Biostat S7:014. doi:10.4172/2155-6180.S7-014
Copyright: © 2012 Yang J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Thermoanaerobacter sp. X514, capable of fermenting most hexose and pentose to produce ethanol, is of critical importance in industrial bioethanol production. This paper provides a detailed investigation of the intracellular metabolic flux flowing activities of X514 under different conditions, including sole glucose substrate condition, sole xylose substrate condition and mixed glucose and xylose substrates condition by means of Gibbs sampling algorithm and stoichiometric metabolic flux balances. Statistical analysis of the results show that all the flux distributions exhibit Gaussian or truncated Gaussian distributions under the assumption that noise of the system is Gaussian distribution. Pentose phosphate pathway becomes more active when xylose is the sole substrate whereas glycolysis pool is more active under sole glucose substrate condition. Major changes of the fluxes activities occur in the connection flux between glycolysis pool and pentose phosphate metabolite pool, indicating the balances between the need for NADPH, the requirement of ATP as well as the necessity of pyruvate under different substrate conditions. Generation of pyruvate reaches its maximum when sole glucose is fed into the system, indicating the preference of the system on glucose consumption. The whole metabolic flux distributions illustrate vivid information about the central metabolic map of X514.