Anti Enterocyte Autoantibodies in Pediatric Celiac Disease
- *Corresponding Author:
- Lerner A
Mikroforum Ring 2
Wendelsheim 55234, Germany
Email: [email protected]
Received date: June 22, 2016; Accepted date: August 05, 2016; Published date: August 16, 2016
Citation: Lerner A, Kushak RI, Jeremias P, Matthias T, Winter HS (2016) Anti Enterocyte Autoantibodies in Pediatric Celiac Disease. J Clin Cell Immunol 7:445. doi:10.4172/2155-9899.1000445
Copyright: © 2016 Lerner A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Many aspects are shared between celiac disease and autoimmune enteropathy: symptoms, diagnosis by serological bio-markers, endoscopic findings, intestinal pathology, differential diagnosis, and pharmaceutical therapy in selected cases. Antibodies to tissue transglutaminase have been described in over 30% of patients with autoimmune enteropathy, but no anti-enterocyte antibodies were detected in a small population of pediatric celiac patients.
The aim of the current study was to identify anti-enterocyte antibodies in pediatric patients with well-characterized celiac disease compared to a group of children with recurrent abdominal pain.
Materials and methods: celiac disease (N=38) was diagnosed based on positive celiac serology (anti-neoepitope tissue transglutaminase (Aesku*) and/or anti endomysial antibodies) and small intestinal biopsy that was consistent with celiac disease.
The comparison group consisted of age and sex matched patients (N=41) with a history of abdominal pain, negative celiac serology, normal upper endoscopy and normal small intestinal histology.
Detection of anti-enterocyte antibody was performed using Western blot. Homogenates from normal human intestinal mucosa were electrophoresed on 7.5% SDS-PAGE and transferred to nitrocellulose membranes. Blots were treated with blinded patient sera and developed using ELISA kit.
Results: In the pediatric celiac group 3/35 (8.6%) compared to 6/35 (17.1%) in the non-celiac group were positive for anti-enterocyte antibody. No statistically significant difference in the presence of anti-enterocyte antibodies in patients with celiac disease.
Conclusions: About 8% of children with celiac disease may have antibodies to enterocytes, but the frequency is not increased when compared to children with recurrent abdominal pain.