Anti-Adipogenic Polyphenols of Water Shield Suppress TNF-ÃÂ±-Induced Cell Damage and Enhance Expression of HAS2 and HAPB2 in Adiponectin- Treated Dermal Fibroblasts
|Hiroshi Shimoda1*, Seikou Nakamura2, Shoketsu Hitoe1, Shuko Terazawa1, Junji Tanaka1, Takahiro Matsumoto2 and Hisashi Matsuda2|
|1Research and Development Division, Oryza Oil & Fat Chemical Co., Ltd., 1 Numata, Kitagata-cho, Ichinomiya, Japan|
|2Kyoto Pharmaceutical University, 1 Shichono-cho, Misasagi, Yamashina-ku, Kyoto 607-8412, Japan|
|Corresponding Author :||Hiroshi Shimoda
Research & Development Division
Oryza Oil & Fat Chemical Co., Ltd.
1 Numata, Kitagata-cho, Ichinomiya, Aichi 493-8001, Japan
E-mail: [email protected]
|Received May 29, 2014; Accepted August 18, 2014; Published August 20, 2014|
|Citation: Shimoda H, Nakamura S, Hitoe S, Terazawa S, Tanaka J, et al. (2014) Anti-Adipogenic Polyphenols of Water Shield Suppress TNF-α-Induced Cell Damage and Enhance Expression of HAS2 and HABP in Adiponectin-Treated Dermal Fibroblasts. Nat Prod Chem Res 2:146. doi: 10.4172/2329-6836.1000146|
|Copyright: © 2014 Shimoda H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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Water shield (Brasenia schreberi J. F. Gmel) is an aquatic plant cultivated in Northern Japan, from which we have isolated polyphenolic compounds including a novel flavonol glycoside, junsainoside A. Recently, dermal fibroblasts have reported to be affected by adipocytokines from subcutaneous adipocytes. Hence, we evaluated the effects of polyphenolic compounds from water shield on fat accumulation, adipocytokine release and adipocytokine-induced fibroblast status. In differentiated 3T3-L1 adipocytes, ethyl gallate, caffeoyl glucose, hypolaetin 7-O-glucoside, kaempferol and junsainoside A significantly suppressed lipid accumulation at 10 μM. In addition to these constituents, ferulic acid, kaempferol 3-O-(6"-galloyl) glucoside, quercetin 3-O-(6"-galloyl) glucoside, quercetin 3-O-glucoside and gossypetin suppressed the lipid accumulation in human subcutaneous adipocytes. Ethyl gallate and junsainoside A also enhanced adiponectin production from subcutaneous adipocytes without induction of TNF-α release. On the other hand, ethyl gallate and quercetin recovered from TNF- α-induced fibroblast damage. Moreover, ferulic acid, quercetin and kaempferol enhanced the expression of hyaluronan binding protein 2 and hyaluronan synthase 2 in adiponectintreated fibroblasts. These results suggested that water shield contains various types of polyphenolic compounds with anti-adipogenic activity, some of which protect dermal fibroblasts from TNF-α damage and probably enhance hyaluronan production. It is assumed that polyphenols in water shield maybe suppress subcutaneous fat accumulation and maintain dermal fibroblast and hyaluronan-related status.