Anti-Aging with High, Selective and Wide Spectrum Anticancer Activities of Jordanian Olive Trees Leaves Special Extracts
Muneer Taleb Abuismail*
Department of Applied Chemical Science, Jordan University of Science and Technology, Irbid, Jordan
- *Corresponding Author:
- Muneer Taleb Abuismail
Department of Applied Chemical Science
Jordan University of Science and Technology
E-mail: [email protected], [email protected]
Received Date: December 10, 2011; Accepted Date: May 28, 2012; Published Date: May 30, 2012
Citation: Abuismail MT (2012) Anti-Aging with High, Selective and Wide Spectrum Anticancer Activities of Jordanian Olive Trees Leaves Special Extracts. J Cancer Sci Ther 4:146-154. doi: 10.4172/1948-5956.1000130
Copyright: © 2012 Abuismail MT. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Olive leaves samples obtained from two representative regions in Jordan and from different olive tree varieties were extracted with ethanol and assayed for their anticancer activity. The brine shrimp lethality bioassay results (LC50) ranged from 0.15 ppm for Roomy cultivar to 305.10 ppm for Shami cultivar from Amman area. Anti tumor activity using cell viability assay showed very low (IC50) arround 100 µg for 3 extracts (Roomy, Roomy Kfary, K18) on 7 Cancer cell lines (breast cancer MCF-7 and MDA-MB-231, skin cancer C32 and MV3, colon cancer SW480, SW620 and HCT 116). Cell cycle analysis and cell death assay using 100 μg of Roomy Kfary extract on selected cell lines (breast cancer MCF-7, skin cancer C32, colon cancer SW420) showed exceptional cancer cells death (apoptosis) after 48 hours (19.16%, 63.86%, 56.78%), respectively. High safety index obtained on normal Fibroblast cells ≥ 100%. At low dosge (25ppm) of extracts on normal fibroblast the percent proliferation became 9 to 10-fold its normal proliferation during 48 hours. These results sugest Jordanian olive cultivars leaves extracts as a powerful anticancer drug.