Antibiofilm Competency of Portunus pelagicus Haemolymph and Identification of its Bioactive CompoundsMahalingam Anjugam1, Arokiadhas Iswarya1, Thiruselvam Indumathi1, Baskaralingam Vaseeharan1*, Raman Pachaiappan2, Narayanan Gopi1 and Palaniyandi Velusamy2
1Crustacean Molecular Biology and Genomics Division, Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Alagappa University, Science Block 4th floor, Burma colony, Karaikudi-630004, Tamil Nadu, India
- Corresponding Author:
- Dr. B. Vaseeharan
Crustacean Molecular Biology
and Genomics Division Biomaterials and
Biotechnology in Animal Health Lab, Department of Animal
Health and Management, Alagappa University, Science Block 4th Floor
Burma Colony, Karaikudi- 630 004, Tamil Nadu, India
Tel: + 91 4565 225682
Fax: + 91 4565 225202
E-mail: [email protected]
Received date: July 20, 2016; Accepted date: August 29, 2016; Published date: August 31, 2016
Citation: Anjugam M, Iswarya A, Indumathi T, Vaseeharan B, Pachaiappan R, et al. (2016) Antibiofilm Competency of Portunus pelagicus Haemolymph and Identification of its Bioactive Compounds. J Aquac Res Development 7:444. doi:10.4172/2155-9546.1000444
Copyright: © 2016 Anjugam M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The marine atmosphere may be investigated as a rich source for novel drugs. In past decades, a number of marine-derived compounds have been isolated and identified. The current effort was made on haemolymph of blue swimmer crab Portunus pelagicus to mark its antimicrobial activity against eight pathogenic bacteria of Gram positive, Gram negative and fungus Candida albicans. Agar well diffusion method was put into practice in deliverance of antibacterial and antifungal activity by measuring zone of inhibition. The findings displays, P. pelagicus haemolymph at the concentration of 150 μl can effectively act against all of challenged bacteria and fungus. Besides, the antibiofilm property of haemolymph against selected bacteria and fungus C. albicans which revealed the potential bactericidal and fungicidal effect at 150 μl concentration of haemolymph. Moreover, the antimicrobial and antibiofilm potent of crab haemolymph was confirmed by growth curve analysis, biofilm growth inhibition and protein leakage assay which collectively conclude the ability of haemolymph in microbial growth inhibition. For the first time, the bioactive compounds were screened from the haemolymph of P. pelagicus through Gas chromatography- Mass spectrometry analysis discloses the existence of 15 types of compounds in haemolymph being in charge for antimicrobial activity.