Antibody Markers for Studying Neurodegeneration in the Nothobranchius Central Nervous SystemT. Genade* and D.M. Lang
Human Biology, Faculty of Health Sciences, University of Cape Town, Private Bag X3, Observatory, 7935, South Africa
- *Corresponding Author:
- Genade T
M.Sc., Human Biology, Faculty of Health Sciences
University of Cape Town, Private Bag X3
Observatory, 7935, South Africa Tel: +27214066553
E-mail: [email protected]
Received date: April 08, 2011; Accepted date: April 29, 2011; Published date: May 02, 2011
Citation: Genae T, Lang DM (2011) Antibody Markers for Studying Neurodegeneration in the Nothobranchius Central Nervous System. J Cytol Histol 2:120. doi:10.4172/2157-7099.1000120
Copyright: © 2011 Genade T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Fish of the genus Nothobranchius have a naturally short lifespan in which they develop several neurological biomarkers of aging. This short lifespan (several months compared to several years in mice and zebrafish) make these fish a promising alternative in aging research. Resveratrol dosing retarded the development of the neurological biomarkers of aging identified in N. furzeri. Resveratrol's method of action was not determined due to the lack of celltype specific markers of degenerative processes. Further research of the Nothobranchius CNS and its degeneration is hampered by the lack of cell-type specific antibodies and other cellular markers of degenerative processes. In this article the authors present a molecular toolbox of five antibodies and a lectin which can be used to study the Nothobranchius central nervous system and its degeneration. These antibodies were tested against western blots of rat, zebrafish and Nothobranchius guentheri brain homogenates as well as frozen sections. Antibodies shown to reliably bind with N. guentheri proteins were: SMI31 which labeled axons and dendrites; mouse anti-human GFAP and rabbit anti-cow GFAP which labeled astroglia; mouse anti-rat tenascin-R which labeled oligodendrocytes; and E587 anti-goldfish L1 which labeled neuronal and astrocytic cell bodies and processes. The BS-I isolectin B4 labeled microglia in the optic nerve and retina. This toolbox will enable researchers to study the aging brain of Nothobranchius as well as its normal development at a cellular level.