alexa Antibody@Silica Coated Iron Oxide Nanoparticles: Synthesis, Capture of E.coli and Sers Titration of Biomolecules with Antibacterial Silver Colloid | OMICS International
ISSN: 2157-7439

Journal of Nanomedicine & Nanotechnology
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Research Article

[email protected] Coated Iron Oxide Nanoparticles: Synthesis, Capture of E.coli and Sers Titration of Biomolecules with Antibacterial Silver Colloid

Shiva K. Rastogi1*, Jamie M. F. Jabal2, Huijin Zhang3, Charlene M. Gibson1, Kevin J. Haler4, You Qiang3, D. Eric Aston2 and A. Larry Branen4

1Department of Chemistry, University of Idaho, Moscow, ID - 83844-2343

2Department of Chemical and Materials Engineering, University of Idaho, Moscow, ID - 83844, USA

3Departmental of Physics and Environmental Science Program, University of Idaho, Moscow, ID - 83844, USA

4Biosensors and Nanotechnology Applications Laboratory, University of Idaho, 1031 N Academic Way, Coeur d’ Alene, ID - 83814, USA

*Corresponding Author:
Shiva K. Rastogi
Department of Chemistry
University of Idaho
Moscow, ID - 83844-2343
Tel: 1-208-758-0416
Fax: 1-208-885-6173
E-mail: [email protected]

Received Date: November 06, 2011; Accepted Date: November 26, 2011; Published Date: November 29, 2011

Citation: Rastogi SK, Jabal JMF, Zhang H, Gibson CM, Haler KJ, et al. (2011) [email protected] Coated Iron Oxide Nanoparticles: Synthesis, Capture of E. coli and Sers Titration of Biomolecules with Antibacterial Silver Colloid. J Nanomedic Nanotechnol 2:121. doi:10.4172/2157-7439.1000121

Copyright: © 2011 Rastogi SK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Silica coated iron oxide (SiO 2 /Fe 3 O 4 + γ- Fe 2 O 3 ) nanoparticles (SIO-NPs; 75±10 nm in diameter) were prepared by encapsulation of iron oxide NPs with silica using sol-gel method and characterized through spectroscopy methods. The SIO NPs were chemically activated by cyanogens bromide and then functionalized with Escherichia coli ( E. coli ) antibodies. These immuno-magnetic (IM NPs) were used to capture and concentrate E. coli from ~ 180 cfu/ mL suspension. The identification of bacteria was performed by plating on nutrient agar, fluorescent microscopy and scanning electron microscopy. Surface enhanced Raman spectroscopy (SERS) was used to identify different biomolecules of bacterial cell upon the interaction of colloidal silver nanoparticle (Ag NPs 6±4 nm) at different period of time. In our previous report we demonstrated the antibacterial property of colloidal Ag NPs. Therefore, current approach, using IM, and Ag NPs and SERS, provide detailed molecular identification of E. coli as Ag NPs interact over the time. This method would be applicable for food safety, environment protection, biological threat material, antibacterial and other routine E. coli identification projects.

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