Anti-Cancer Drug Screening Based on a Adipose-Derived Stem Cell/Hepatocyte 3D Printing TechniqueXinru Zhao1, Siqi Du1, Lei Chai1, Yufan Xu1, Libiao Liu1, Xinwei Zhou1, Jiayin Wang1, Weiming Zhang1, Cheng-Hsien Liu2, Xiaohong Wang1,3*
- *Corresponding Author:
- Xiaohong Wang
Department of Mechanical Engineering
Tsinghua University, Beijing 100084
E-mail: [email protected]
Received date: February 11, 2015; Accepted date: April 06, 2015; Published date: April 08, 2015
Citation: Zhao X, Du S, Chai L, Xu Y, Liu L, et al. (2015) Anti-Cancer Drug Screening Based on a Adipose-Derived Stem Cell/Hepatocyte 3D Printing Technique. J Stem Cell Res Ther 5:273. doi:10.4172/2157-7633.1000273
Copyright: ©2015 Zhao X, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: During the last several decades, drug screening results based on two-dimensional (2D) cell cultures could hardly be duplicated by animal experiments due to different cell growth environments. With the development of three-dimensional printing (3DP) techniques, in vitro 3D cell cultures show great advantages in many areas. In this study, a 3D drug screening model based on a cell-laden gelatin/alginate/fibrinogen hydrogel was established using a cell 3D printer developed in Tsinghua University.
Methods: 2D and 3D drug screening effects were compared with three anti-liver tumor drugs (i.e. astragalus polysaccharide, 5-Fluorouracil and matrine). Cell survival rates were tested using 3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyl tetra-sodium bromide colorimetric and cell counting kit-8 methods. Some other detection methods, such as 4',6-diamidino-2-phenylindole/5- or 6-(N-Succinimidyloxycarbonyl)-3',6'-O,O'-diacetylfluorescein staining, acridine orange/propidium iodide staining, hematoxylin-eosin staining and scanning electron microscope, were applied to assist the analyses.
Results: Cell viability of the 3D adipose-derived stem cell/hepatocyte cocultures was increased. The 3D model enhanced the drug-resistance of hepatocytes.
Conclusion: This in vitro 3D model has promise to be widely used in drug screening.