alexa Anti-cancer Effects of Glypican-3 on Huh-7 Hepatocellular Carcinoma Cells | OMICS International | Abstract
ISSN: 2157-7013

Journal of Cell Science & Therapy
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Research Article

Anti-cancer Effects of Glypican-3 on Huh-7 Hepatocellular Carcinoma Cells

Jiyu Wen1, Xiaojun Wen2, Jinju Wang3, Yang Shu2, Zhidong Qiu2, Zhongkao Liu2, Ran Li2, Guofang Zeng2, Shiting Bao2, Huilai Miao2, Yanfang Chen2,3* and Mingyi Li2*

1Department of Oncology, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, China

2Department of Hepatobiliary Surgery, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, China

3Department of Pharmacology and Toxicology, Wright State University Boonshoft School of Medicine, Dayton, OH, USA

*Corresponding Author:
Yanfang Chen
Department of Pharmacology and Toxicology
Wright State University Boonshoft School of Medicine
Dayton, OH, USA, 45435
Tel: 937-775-3265
Fax: 937-775-7221
E-mail: [email protected]
Ming-Yi Li
Affiliated Hospital of Guangdong Medical College
Zhanjiang, Guangdong, P.R China, 52400
Tel: 86-759-238-7417
Fax: 86-759-238-7685
E-mail: [email protected]

Received Date: October 17, 2014; Accepted Date: November 21, 2014; Published Date: November 24, 2014

Citation: Wen J, Wen X, Wang J, Shu Y, Qiu Z, et al. (2014) Anti-cancer Effects of Glypican-3 on Huh-7 Hepatocellular Carcinoma Cells. J Cell Sci Ther 5:186. doi: 10.4172/2157-7013.1000186

Copyright: © 2014 Wen J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Aim: Previous studies have suggested Glypican-3 (GPC3) could be a valuable diagnostic marker for hepatocellular carcinoma. This study examined the effects of overexpression of GPC3 on Huh-7 hepatoma cells.

Methods: We constructed a recombinant plasmid vector pcDNA3.1 (+)-GPC3 for GPC3 overexpression studies in Huh-7 cells. RT-PCR and Western blotting were used to confirm GPC3 gene expression. Cell proliferation was evaluated by 5-ethynyl-2-deoxyuridine (EdU) incorporation assay. Cell cycle progression and apoptosis were determined by flow cytometry using propidium iodide (PI) and Annexin V-FITC/PI staining, respectively. Cell migration and invasion were examined by Boyden Transewll and Matrigel assays.

Results: GPC3 overexpression effectively inhibited proliferation, induced cell cycle arrest at S phase and increased apoptosis in Huh-7 cells. Furthermore, GPC3 overexpression significantly inhibited the migration and invasion ability of Huh-7 cells. Conclusion: Our results demonstrate that GPC3 could be a new therapeutic target for hepatocellular carcinoma

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