alexa Anti-Inflammatory Activity of Quantum Energy Living Body on Lipopolysaccharide-Induced Murine RAW 264.7 Macrophage Cell Line

Bioceramics Development and Applications
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Research Article

Anti-Inflammatory Activity of Quantum Energy Living Body on Lipopolysaccharide-Induced Murine RAW 264.7 Macrophage Cell Line

Jong Doo Lee1, Emil Joseph S Vergara2, Soo Hee Choi2, Seong Gu Hwang2* and Gun Woong Bahng3

1Quantum Energy Research Center, An-Yang, Kyung Ki Do, 431-804, Republic of Korea

2Department of Animal Life and Environmental Science, Hankyong National University, Korea

3QELBY Research Institute, Department of Technology and Society, State University of New York, Korea (SUNY Korea), Incheon, 406-840, Republic of Korea

Corresponding Author:
Seong Gu Hwang
Department of Animal Life and Environmental Science,
Hankyong National University, Korea
Tel: +82-10-3213-3808
Fax: +82-31-670-5121
E-mail: [email protected]

Received date: November 20, 2015; Accepted date: January 14, 2016; Published date: January 25, 2016

Citation: Lee JD, Vergara EJS, Choi SH, Hwang SG, Bahng GW (2016) Anti- Inflammatory Activity of Quantum Energy Living Body on Lipopolysaccharide-Induced Murine RAW 264.7 Macrophage Cell Line. Bioceram Dev Appl 6:089. doi:10.4172/2090-5025.1000089

Copyright: © 2016 Lee JD, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Recent developments from science and medical science show a growing interest in the anti-inflammatory activity of natural materials. Inflammation is the body’s physiologic response to injurious stimulation and is known to be mediated by various pro-inflammatory cytokines (e.g. TNF-α, IL-1β, IL-6 etc) and iNOS (inducible nitric oxide synthase). Quantum energy living body (QELBY) powder is a fusion of a special ceramic powder with natural clay mineral classified as quantum energy radiating material (QERM). The powder, composed mostly of silicon dioxide, is known to radiate reductive radiant energy. This study was designed to evaluate the anti-inflammatory activities of QELBY powder on RAW 264.7 mouse macrophage cells. QELBY powder was mixed with DMEM media and was allowed to stand for 48 hours. Afterwards, the supernatant was taken and diluted to various concentrations (0,5,10,20,40 μg/ml) prior to use. CCK-8 assay was done to determine the effects on cell viability. In addition, NO assay performed to elucidate the effect of QELBY on the NO production of LPS-stimulated macrophages. Lastly, RT-PCR and Western blot analysis for the detection of the mRNA and protein expressions, respectively, of proinflammatory cytokines and iNOS was made. Results demonstrated that QELBY powder causes both an increase in cell proliferation and a concentration-dependent decrease in NO production. Moreover, the mRNA and protein expressions of pro-inflammatory cytokines and iNOS were also inhibited. Taken together, these show that QELBY powder has anti-inflammatory activity and could therefore be used further in the development of materials that induce such kinds of benefits.


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