Antimicrobial Activity of Endophytic Fungal Isolate in Argemone Maxicana;A Traditional Indian Medicinal Plant
Shiv kumar verma1, Anand kumar2, Ashish3, Mira debnath (das)*
Research scholar, School of Biochemical Engineering, Indian Institute of Technology (BHU), Varanasi, India
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The study was carried out to screen and isolate endophytic fungi, showing antimicrobial activity, from Root and stem tissues of Argemone maxicana (family papaveraceae). A total of fourteen Endophytic fungi, based on morphological characteristics, were isolated (assigned as AMS1- AMS8 for stem isolates and AMR9-AMR14 for root isolates). Among these fourteen isolates, AMS5 was found to show maximum antimicrobial activity, in compare to other isolates, against gram positive, gram negative bacteria and fungi. The antimicrobial activity was tested against Escherichia coli. Pseudomonas aeroginosa, Bacillus subtilis, Staphylococcus aureus, Ralstonia solanacearum, X.oryzae Penicillium chrysogenum, Candida albicans, Phoma exigua, Sclerotium rolfsii and Sclerotinia scleratiourum. Minimum inhibitory concentration (MIC) of crude extract against test microorganisms was determined. FTIR analysis of crude extract was carried out for functional group.