Antimicrobial Behavior of Intracellular Proteins from Two Moderately Halophilic Bacteria: Strain J31 of Terribacillus halophilus and Strain M3- 23 of Virgibacillus marismortui
Badiaa Essghaier*, Cyrine Dhieb, Hanene Rebib, Saida Ayari, Awatef Rezgui Abdellatif Boudabous and Najla Sadfi-Zouaoui
Microorganisms and Active Biomolecules Laboratory, Faculty of Sciences of Tunis, University Campus, Tunisia
- *Corresponding Author:
- Badiaa Essghaier
Microorganisms and Active Biomolecules Laboratory
Faculty of Sciences of Tunis
University Campus, Tunisia
E-mail: [email protected]
Received date: December 07, 2013; Accepted date: December 29, 2013; Published date: January 02, 2014
Citation: Essghaier B, Dhieb C, Rebib H, Ayari S, Boudabous ARA, et al. (2014) Antimicrobial Behavior of Intracellular Proteins from Two Moderately Halophilic Bacteria: Strain J31 of Terribacillus halophilus and Strain M3-23 of Virgibacillus marismortui. J Plant Pathol Microb 5:214. doi:10.4172/2157-7471.1000214
Copyright: © 2014 Essghaier B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In the present study, we firstly aimed to determine the ability of halophilic bacteria to improve tomato growth. as well as to detect the antimicrobial activities from two moderately halophilic bacteria strain M3-23 of Virgibacillus marismortui and strain J31 of Terribacillus halophilus exhibited by their intracellular proteins. The results showed that both bacteria were able to improve stem tomato growth by comparison of untreated tomato. The halophilic bacteria were also able to produce intracellular antifungal enzyme: glucanase produced by V. marismortui (1.74U/mg) and chitinase (39.39U/mg) produced by T. halophilus. Both chitinases were halotolerants (active in the presence of (0% to 30% NaCl (w/v)). Chitinase produced by strain J31 was alcaline (pH optimum pH 12), but chitinase from strain M3- 23 was acidic (pH 4 optimum) more than 90% and 80% of activities were retained in the presence of pH value from 4 to 12, respectively for strain J31 and M3-23. Both enzymes were thermotolerants; optimum temperature was 80°C and 90°C respectively for strain J31 and strain M3-23. Both strains have lysozyme activity and value ranging from 6.6 U/ml to 6.8 U/ml respectively for strain J31 and strain M3-23. On the whole, the most potent in vitro antifungal effect was demonstrated by intracellular compound produced by strain J31 compared to strain M3-23. This study, was the first showing the antimicrobial efficiency of moderately halophilic bacteria by means of their intracellular compounds, by means of the spore germination reduction and the destroy of mycelial growth of Botrytis cinerea, in vitro. The distinguishable characteristics of their intracellular halotolerant and thermotolerant chitinases make them as good candidates for biotechnological applications.