alexa Antimicrobial Potency of Methanolic Leaf Extracts from Selected Medicinal Plants against Staphylococcus aureus
ISSN: 2161-0703

Journal of Medical Microbiology & Diagnosis
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Research Article

Antimicrobial Potency of Methanolic Leaf Extracts from Selected Medicinal Plants against Staphylococcus aureus

Rachuonyo HO1*, Ogola PE2, Arika WM2, Kiboi NG2 and Wambani JR3

1Department of Microbiology, Kenyatta University, Nairobi, Kenya

2Department of Biochemistry and Biotechnology, Kenyatta University, Nairobi, Kenya

3Department of Medical Laboratory Sciences, Kenyatta University, Nairobi, Kenya

*Corresponding Author:
Rachuonyo HO
Department of Microbiology
Kenyatta University
P.O Box 43 844-00100 Nairobi, Kenya
Tel: 254 20 870 4000
E-mail: [email protected]

Receaved date: Dec 24, 2015; Accepted date: Feb 13, 2016; Published date: Feb 18, 2016

Citation: Rachuonyo HO, Ogola PE, Arika WM, Kiboi NG, Wambani JR (2016) Antimicrobial Potency of Methanolic Leaf Extracts from Selected Medicinal Plants against Staphylococcus aureus. J Med Microb Diagn 5:219. doi: 10.4172/2161-0703.1000219

Copyright: © 2016 Rachuonyo HO, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



The main aim of the study was to test for the antimicrobial potency of Aloe secundiflora, Bulbine frutescens, Tagetes minuta and Vernonia lasiopus against Staphylococcus aureus. All the plants showed a pronounced antimicrobial activity against Staphylococcus aureus with Tagetes minuta being the most active at low concentrations (MIC 8.9 mg/ml; MBC 10.0 mg/ml) whereas Vernonia lasiopus showing less activity (MIC 12.2 mg/ml; MBC 14.2 mg/ml). The efficacy test was carried out using the disc diffusion method. The standard antibiotics used were ciprofloxacin (5 μg/ml) and vancomycin (3 μg/ml) showed significant antimicrobial activity by producing zones of inhibition of 22 mm and 25 mm respectively. Dimethyl sulphoxide and distilled water were used as negative control. The extracts from the plants were also screened for the presence of phytochemicals with the results showing the presence of flavonoids, alkaloids, tannins and saponins in all the extracts. The study suggested that the selected medicinal plants can be used effectively in the treatment of bacterial infection caused by Staphylococcus aureus.


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