Antinociceptive and Anti-Inflammatory Activities of Euphorbia Hirta L. Leaves in Animal Models.
The present study was done to arrive the potential of chloroform extract of Euphorbia (E) Hirta L. (Family-Euphorbiaceae) leaves on antinociceptive, behavioral study and anti-inflammatory effects using various animal models. The dried, powdered leaves of, Euphorbia Hirta L. were extracted successively with petroleum ether (60-80°C) and chloroform in soxhlet apparatus. The chloroform extract (yield 5.47% w/w with respected to dry powdered plant material) was selected for all experimental procedure. Two models were taken to derive the effects of nociception, by the tail immersion and hot plate method on Swiss albino mice and anti inflammatory effect were investigated by employing the carrageenan induced rat paw edema test in adult Wister albino rats. Behavioral study was investigated by elevated plus maze method in Swiss albino mice. Results were revealed that the EHCE has significant antinociceptive effect (P < 0.001) at the dose levels of 100, 200 and 400 mg/kg, orally in mice and also produced anti-inflammatory effect (P < 0.001) at the same dose levels used in the rats. Behavioral study of the EHCE shows that there is no significant anxiolysis effect when used orally. It concludes that, EHCE possessed remarkable antinociceptive effect and anti-inflammatory effect but no anxiolytic effect on animal models.