Antioxidant Activity of White (Morus alba L.) and Black (Morus nigra L.)Berries against CCl4 Hepatotoxic Agent
Background and Aim: Our research investigates black, white and mixture of fresh berries role in liver injury caused by CCl4 through biochemical parameters and histopathological determinations.
Methods: Using berry as preventing or caring agents in comparing with treating or relieving or might curing agents against CCl4 hepatotoxicity. Injection with CCl4 was in the second 10 days, while preventing showed that feeding with berry on the first 10 days and relieving was feeding with berry on the third 10 days.
Results: Preventing infected rats with white berry increased weight (17%), food efficiency ratio FER (6%), decreased aspartate aminotransferase (AST) (81%), urea (25.5%) comparing to infected rat group. Risk factor in lipid profile showed improvement by preventing black, white followed by mixture berries (26-58%) compared with CCl4 group. Preventing with black, white then mixture berry showed significant improvement for risk factor. Generally, optimistic data has been found for preventing black berry in malondialdehyde (MDA) and fucosidase values in the final stage. In the first stage, preventing white and mixture fresh berry showed significant increase for antioxidant and fucosidase activity, respectively. Histopathological profiles indicated that relieving black berry and protected mixture were much enhanced than protected black and relieving mixture, respectively.
Conclusion: Carbon tetrachloride is showing infection and hepatotoxicity in liver diseases due to its reactive intermediates. White, black and their equal mixture in the diet of Sprague-Dawley rats showed protective and even relieving for CCl4 toxicity.