alexa Antioxidant, Glucose Uptake Stimulatory, α-Gluco
ISSN: 2329-6836

Natural Products Chemistry & Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Antioxidant, Glucose Uptake Stimulatory, α-Glucosidase and α-Amylase Inhibitory Effects of Myrianthus arboreus Stem Bark

Harley BK1*, Dickson RA1 and Fleischer TC2

1Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, KNUST, Kumasi, Ghana

2Department of Herbal Medicine, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, KNUST, Kumasi, Ghana

Corresponding Author:
Harley BK
Faculty of Pharmacy and Pharmaceutical Sciences College of Health Sciences
Department of Pharmacognosy, Kwame Nkrumah University of Science
and Technology, Kumasi Ashanti Region, Ghana
Tel: +2330265398208
E-mail: [email protected]

Received date: May 16, 2017; Accepted date: May 25, 2017; Published date: May 30, 2017

Citation: Harley BK, Dickson RA, Fleischer TC (2017) Antioxidant, Glucose Uptake Stimulatory, α-Glucosidase and α-Amylase Inhibitory Effects of Myrianthus arboreus Stem Bark. Nat Prod Chem Res 5:273. doi:10.4172/2329-6836.1000273

Copyright: © 2016 Mbamalu ON, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Myrianthus arboreus is used traditionally in the management of diabetes in Ghana. The study seeks to evaluatethe antioxidant, glucose uptake, α-glucosidase and α-amylase inhibitory activities of the stem bark to support its folkloric use. The ethanol stem bark extract of M. arboreus was partitioned successively with petroleum ether, chloroform, ethyl acetate and n-butanol. The extract and fractions were evaluated for their glucose uptake effects in C2C12 myotubes and 3T3-L1 adipocytes. Their inhibitory action on α-glucosidase and α-amylase were also investigated whereas their antioxidant activities were evaluated using the DPPH and nitric oxide scavenging assays. The crude extract was further evaluated for its phenolic content, antioxidant capacity and reducing power. The stem bark extract significantly (P<0.05) stimulated glucose uptake in C2C12 myotubes and 3T3-L1 adipocytes when compared to control and also showed potent inhibitory activities against α-glucosidase and α-amylase with 11-fold and 5-fold better activity than acarbose respectively. The DPPH and nitric oxide radical scavenging effect of the extract were comparable with the ascorbic acid and α-tocopherol respectively. The extract had high phenol content with tannic acid equivalent of (114.70 ± 10.48) mg/g with an ascorbic acid equivalent of (98.34 ± 14.50) mg/g. The fractions demonstrated varying glucose uptake and enzyme inhibitory activities with the ethyl acetate fraction being the most potent. The ethyl acetate fraction demonstrated the highest scavenging effect on the DPPH and nitric oxide radicals. M. arboreus stem bark extract showed significant antioxidant, glucose uptake and enzyme inhibitory activities justifying its use in traditional medicine.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords