Antioxidant Properties of Polysaccharide Extract from Juice and Pomace of Psidium guajava (Pink Guava) and its Mitigating Effect in Early Stage of Atherogenesis
- *Corresponding Author:
- Ihsan Safwan Kamarazaman
Bioactivity Programme, Natural Products Division
Forest Research Institute of Malaysia (FRIM)
52109 Kepong, Selangor, Malaysia
E-mail: [email protected]
Received Date: July 26, 2017 , 2017; Accepted Date: August 10, 2017, 2017; Published Date: August 15, 2017
Citation: Kamarazaman IS, Abdul RL, Nik Hasan MK, Ahmad MR, Ayob MKM, et al. (2017) Antioxidant Properties of Polysaccharide Extract from Juice and Pomace of Psidium guajava (Pink guava) and its Mitigating Effect in Early Stage of Atherogenesis. Nat Prod Chem Res 5:273. doi: 10.4172/2329-6836.1000273
Copyright: © 2017 Kamarazaman IS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In this study, the polysaccharide extracts of Pink guava juice (PGJ) and pomace (PGP) were characterised and their antioxidant properties and mitigating effect on expression level of biomolecules involved in early stage of atherosclerosis were investigated. Chemical characterization were performed by HPLC and it was revealed that molar weight of PGJ and PGP by percentage (%) were 63.0:16.2:9.9:6.4:4.2:0.1% of galacturonic acid, xylose, glucose, mannose, galactose and rhamnose, respectively while PGP has 72.6:4.7:2.7:1.3% of galacturonic acid, glucose, galactose and xylose, respectively. PGJ was found to has moderate antioxidant activity whereby PGP has more potent effect at lower concentration. Both PGJ and PGP have the ability to inhibit lipid peroxidation in dose dependent manner. Both also attenuate the tumor necrosis factor (TNF)-α induced ICAM-1 and VCAM-1 expression in dosedependent manner but only PGP could attenuate the MCP-1 expression.