Anti-Oxidative Stress and Anti-Apoptosis Effects of He Ying An Xin- FormulaHe Wei1#, An Yating2#, Li Yongmin1*, Zhang Haixia1, Wang Lei2, Chen Xilong1 and Su Zhiyuan1
- *Corresponding Author:
- Li Yongmin
Hebei North University, 11 Zuanshi South Road
Jingkai District, Hebei, 075000, China
E-mail: [email protected] sina.com
Received date: January 18, 2016; Accepted date: February 06, 2016; Published date: February 12, 2016
Citation: Wei H, Yating A, Yongmin L, Haixia Z, Lei W, et al (2016) Anti-Oxidative Stress and Anti-Apoptosis Effects of He Ying An Xin-Formula. J Pharmacogn Nat Prod 2:115. doi: 10.4172/2472-0992.1000115
Copyright: © 2016 Wei H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Objective: To investigate anti-oxidative protective effects and potential anti-apoptosis mechanisms of He Ying An Xin-Formula (HYAX-F). Method: VSMC were incubated by H2O2 (200 μmol/L) for 2 h as oxidative stress control group, same dosage of H2O2 was administered 2 hours after treatment with HYAX-F (100 g/mL, 50 g/mL, 25 g/mL) as therapeutic group and incubated for another 24 hours, cell supernant content of GSH and MDA were measured. Sub-cutaneous injection in back with D-galactose (125 mg/kg) to establish ageing rat model. Control group: D-galactose125 mg/kg, therapeutic groups: HYAX-F 500 mg/kg+D-galactose 125 mg/kg, HYAX-F 250 mg/kg+D-galactose 125 mg/kg, HYAX-F 125 mg/kg+D-galactose 125 mg/kg, normal group and young group. The RT-PCR technique was used to measure the expression of relevant genes, such as tumor necrosis factor- alpha (TNF-α) and B-cell lymphoma-2(Bcl-2) in the tissues of brain and liver. Results: Compared with the control group, HYAX-F can significantly enhance the content of GSH (P<0.05) and decrease the content of MDA (P<0.01). Also HYAX-F can significantly decrease TNF-α expression and increase Bcl- 2 expression in rats brain and liver tissues (P<0.05, P<0.01). Conclusion: HYAX-F has greatly protective effects on anti-oxidative of rat VSMC and anti-apoptosis of ageing rats.