alexa Anti-Oxidative Stress and Anti-Apoptosis Effects of He Ying An Xin-Formula | OMICS International| Abstract

ISSN: 2472-0992

Journal of Pharmacognosy & Natural Products

  • Research Article   
  • J Pharmacogn Nat Prod 2016, Vol 2(1): 1
  • DOI: 10.4172/2472-0992.1000115

Anti-Oxidative Stress and Anti-Apoptosis Effects of He Ying An Xin-Formula

He Wei1#, An Yating2#, Li Yongmin1*, Zhang Haixia1, Wang Lei2, Chen Xilong1 and Su Zhiyuan1
1Hebei North University, Zhangjiakou, China
2Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin, China
#Contributed equally to this work
*Corresponding Author : Li Yongmin, Hebei North University, 11 Zuanshi South Road, Jingkai District, Hebei, 075000, China, Tel: 0086-313-4029228, Email: [email protected]

Received Date: Jan 18, 2016 / Accepted Date: Feb 06, 2016 / Published Date: Feb 12, 2016

Abstract

Objective: To investigate anti-oxidative protective effects and potential anti-apoptosis mechanisms of He Ying An Xin-Formula (HYAX-F).

Method: VSMC were incubated by H2O2 (200 μmol/L) for 2 h as oxidative stress control group, same dosage of H2O2 was administered 2 h after treatment with HYAX-F (100 g/mL, 50 g/mL, 25 g/mL) as therapeutic group and incubated for another 24 h, cell supernant content of GSH and MDA were measured. Sub-cutaneous injection in back with D-galactose (125 mg/kg) to establish ageing rat model. Control group: D-galactose 125 mg/kg, therapeutic groups: HYAX-F 500 mg/kg+D-galactose 125 mg/kg, HYAX-F 250 mg/kg+D-galactose 125 mg/kg, HYAX-F 125 mg/kg+D-galactose 125 mg/kg, normal group and young group. The RT-PCR technique was used to measure the expression of relevant genes, such as tumor necrosis factor- alpha (TNF-α) and B-cell lymphoma-2(Bcl-2) in the tissues of brain and liver.

Results: Compared with the control group, HYAX-F can significantly enhance the content of GSH (P<0.05) and decrease the content of MDA (P<0.01). Also HYAX-F can significantly decrease TNF-α expression and increase Bcl- 2 expression in rats brain and liver tissues (P<0.05, P<0.01).

Conclusion: HYAX-F has greatly protective effects on anti-oxidative of rat VSMC and anti-apoptosis of ageing rats.

Keywords: He Ying An Xin-Formula (HYAX-F); Vascular smooth muscle cells; Anti-oxidative stress; Ageing rats; Anti-apoptosis

Citation: Wei H, Yating A, Yongmin L, Haixia Z, Lei W, et al. (2016) Anti-Oxidative Stress and Anti-Apoptosis Effects of He Ying An Xin-Formula. J Pharmacogn Nat Prod 2: 115. Doi: 10.4172/2472-0992.1000115

Copyright: ©2016 Wei H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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