Anti-Oxidative Stress and Anti-Apoptosis Effects of He Ying An Xin-Formula
Received Date: Jan 18, 2016 / Accepted Date: Feb 06, 2016 / Published Date: Feb 12, 2016
Objective: To investigate anti-oxidative protective effects and potential anti-apoptosis mechanisms of He Ying An Xin-Formula (HYAX-F).
Method: VSMC were incubated by H2O2 (200 μmol/L) for 2 h as oxidative stress control group, same dosage of H2O2 was administered 2 h after treatment with HYAX-F (100 g/mL, 50 g/mL, 25 g/mL) as therapeutic group and incubated for another 24 h, cell supernant content of GSH and MDA were measured. Sub-cutaneous injection in back with D-galactose (125 mg/kg) to establish ageing rat model. Control group: D-galactose 125 mg/kg, therapeutic groups: HYAX-F 500 mg/kg+D-galactose 125 mg/kg, HYAX-F 250 mg/kg+D-galactose 125 mg/kg, HYAX-F 125 mg/kg+D-galactose 125 mg/kg, normal group and young group. The RT-PCR technique was used to measure the expression of relevant genes, such as tumor necrosis factor- alpha (TNF-α) and B-cell lymphoma-2(Bcl-2) in the tissues of brain and liver.
Results: Compared with the control group, HYAX-F can significantly enhance the content of GSH (P<0.05) and decrease the content of MDA (P<0.01). Also HYAX-F can significantly decrease TNF-α expression and increase Bcl- 2 expression in rats brain and liver tissues (P<0.05, P<0.01).
Conclusion: HYAX-F has greatly protective effects on anti-oxidative of rat VSMC and anti-apoptosis of ageing rats.
Keywords: He Ying An Xin-Formula (HYAX-F); Vascular smooth muscle cells; Anti-oxidative stress; Ageing rats; Anti-apoptosis
Citation: Wei H, Yating A, Yongmin L, Haixia Z, Lei W, et al. (2016) Anti-Oxidative Stress and Anti-Apoptosis Effects of He Ying An Xin-Formula. J Pharmacogn Nat Prod 2: 115. Doi: 10.4172/2472-0992.1000115
Copyright: ©2016 Wei H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Select your language of interest to view the total content in your interested language
Share This Article
October 18-20, 2018 Warsaw, Poland
- Total views: 8351
- [From(publication date): 2-2016 - May 21, 2018]
- Breakdown by view type
- HTML page views: 8256
- PDF downloads: 95