Anti-Rhinovirus Activity of Ethyl 4-(3-(2-(3-Methylisoxazol-5-Yl) Ethoxy) Propoxy) Benzoate (EMEB)
Department of Biology, Center for Biotechnology, Sbarro Institute for Cancer Research and Molecular Medicine, Temple University, Philadelphia, USA
- *Corresponding Author:
- Giulio Tarro
Department of Biology, Center for Biotechnology
Sbarro Institute for Cancer Research and Molecular Medicine
Temple University, Philadelphia, USA
Tel: (+39) 0815750090
E-mail: [email protected]
Received date: February 18, 2016 Accepted date: March 15, 2016 Published date: March 18, 2016
Citation: Tarro G (2016) Anti-Rhinovirus Activity of Ethyl 4-(3-(2-(3-Methylisoxazol- 5-Yl) Ethoxy) Propoxy) Benzoate (EMEB). Pharm Anal Acta 7:469. doi:10.4172/2153-2435.1000469
Copyright: © 2016 Tarro G. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The compuond EMEB has got a definite anti-Rhinovirus activity on both HRV14 (group A) and HRV39 (group B). The specific activity is lower than that found for Pirodavir used as a positive control, but, since the cytotoxic activity of EMEB on human HeLa cells is more favourable than that of Pirodavir (50 μg/ml against 3 μg/ml), the final Protection Index is higher for EMEB (> 700) as compared to Pirodavir (250). EMEB seems to be stable in aqueous solutions, since its activity after 10 days was unchanged. When EMEB is challanged with Rhinovirus infected HeLa cells during the whole reproduction cicle, its antiviral activity remains evident and strong even after 18 hours from infection. This fact is important because it means that the compound keeps functioning even when the viral infection is already in progress; this finding makes us to hypothesize that the compound EMEB could act not only as a prophylactic agent against the common cold, but also as a therapeutic drug in patients who already show the disease symptoms (at least within the first 24 hours from the start of symptoms). These last statements must be confirmed with assays on the mechanism of action of the compound, by analysing its adhesion to the cell virus internalization into the cells, the viral uncoating, transcription and translation, and finally on viral morphogenesis.