Apatitic Deposition in Osteoblasts Cultured on Micropatterned Silicon
- *Corresponding Author :
- Yizhi Meng
Department of Materials Science and Engineering
Stony Brook University, Stony Brook, NY, 11794-2275, USA
Received date: 16 January 2014; Accepted date: 4 June 2014; Published date: 13 June 2014
Citation: Kathryn D, Hadjiargyrou M, Yizhi M (2014) Apatitic Deposition in Osteoblasts Cultured on Micropatterned Silicon. Bioceram Dev Appl 4:075. doi:10.4172/2090-5025.1000075
Copyright: © 2014 Kathryn Dorst et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In this study, the osteogenic differentiation of murine pre-osteoblasts cultured on patterned silicon was investigated. Specifically, micropatterns were fabricated in <100> orientation silicon wafers to create linear gratings with a width of either 2 μm (2 μm depth, 10 μm pitch) or 20 μm (2 μm depth, 30 μm pitch). Two subclones (4, 24) of the MC3T3-E1 osteoblast-like cell line were seeded on the micropatterns and cultured in osteogenic induction medium for 28 days. Cells cultured on planar silicon served as the controls. Apatite formation was examined using synchrotron X-ray diffraction. Deposition of  and / apatite-like material was clearly observed in subclone 4 (strongly mineralizing) osteoblasts cultured on both micro patterns.