Apolipoprotein(a) Size and Lipoprotein(a) Concentrations in Patients with Good and Poor Coronary Collateral Flow - an Interrelation Discovered by Proteomic Screening of Pooled Plasma Samples
Laboratory of Thrombosis Research, Department of Clinical Research, University of Bern, 3010 Bern, Switzerland
- *Corresponding Author:
- Dr. Manfred Heller
Laboratory of Thrombosis Research
Department of Clinical Research
University of Bern
3010 Bern, Switzerland
Received Date: August 20, 2008; Accepted Date: October 17, 2008; Published Date: November 05, 2008
Citation: Stalder D, Cung T, Gloekler S, Meier P, Schlappritzi E, et al. (2008) Apolipoprotein(a) Size and Lipoprotein(a) Concentrations in Patients with Good and Poor Coronary Collateral Flow-an Interrelation Discovered by Proteomic Screening of Pooled Plasma Samples. J Proteomics Bioinform 1: 389-400. doi: 10.4172/jpb.1000048
Copyright: © 2008 Stalder D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
We discovered and validated medium sized
(a) as a marker for good myocardial collaterization. A total of 80 subjects were investigated in two serial studies: a discovery study (n=14) applying a pooling strategy to a gel and label free proteomics platform followed by a validation study (n=80) measuring apolipoprotein(a) isoforms and concentration in individual subjects. Degree of myocardial collaterization as well as apolipoprotein(a) concentration and isoform determination were performed by state-of-the-art methodologies. As apolipoprotein(a) concentration negatively correlates with isoform size (variable number of Kringle-IV type 2 repeats in human population), subjects were grouped into patients with small, medium and large apolipoprotein(a) isoforms for the statistical analysis. Among the 70 subjects with medium and large apolipoprotein(a) isoforms (>17 Kringle-IV type 2 repeats), subjects with insufficient collaterization (n=57) had a median apolipoprotein(a) concentration of 11.9 nmol/L, while patients with sufficient collaterization (n=13) had a median concentration of 31.3 nmol/L (p=0.033, Mann-Whitney U-test). Among the 52 subjects with medium sized apolipoprotein(a) isoforms (30< Kringle IV type 2 repeats >17) the difference in concentration was even more significant (13.4 vs 33.5 nmol/L, p=0.008).