Apoptotic Index and Mib-1 Antibody Expression in Premalignant and Malignant Lesions of Uterine CervixKanupriya Gupta1*, Kiran Alam1, Veena Maheshwari1, Roobina Khan1 and Rajyashri Sharma2
- *Corresponding Author:
- Dr. Kanupriya Gupta
Department of Pathology, J.N. Medical College
AMU Aligarh, Haryana, India
E-mail: [email protected]
Received date: August 23, 2013; Accepted date: September 20, 2013; Published date: September 22, 2013
Citation: Gupta K, Alam K, Maheshwari V, Khan R, Sharma R (2013) Apoptotic Index and Mib-1 Antibody Expression in Premalignant and Malignant Lesions of Uterine Cervix. Gynecol Obstet 3:173. doi: 10.4172/2161-0932.1000173
Copyright: © 2013 Gupta K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Introduction: Cervical cancers are the second most frequent type of female cancer, responsible for about 5% of cancer deaths in females worldwide. Recently, parameters of cell proliferation and cell death have emerged as important diagnostic and prognostic tools.
Aims: The aim was to evaluate the role of Apoptotic Index and Ki-67 as proliferation marker in premalignant and malignant lesions of uterine cervix. Materials and Methods: The study included 179 patients of cervical dysplasias and malignancy. Evaluation of Apoptotic Index (using light microscopy) was performed on hematoxylin and eosin-stained sections. Ki-67 (MIB-1 antibody) expression was both graded as well as Labelling Index was calculated. Statistical evaluation was carried out using the Student t test (p<0.05).
Results: There was increase in mean Apoptotic Index with increasing grade of dysplasia and difference in mean values between CIN-I and CIN-II; CIN-I and CIN-III were found to be statistically significant. Also Apoptotic Index increased from well differentiated Squamous Cell Carcinoma (SCC) to poorly differentiated SCC. There was increase in mean Labelling Index with increasing grade of dysplasia and when the p value amongst these groups was statistically significant. Labelling Index was maximum in Poorly Differentiated SCC and minimum in Moderatly Differentiated SCC and p value amongst these groups was found to be statistically significant.
Conclusion: Both Apoptotic Index and Ki-67 expression could be used as a biomarkers in the evaluation of the proliferative activity and progressive potential of dysplastic and neoplastic changes.