alexa Application of a Novel High Yield Volume-Reduction Method for the Cryopreservation of 950 UC Blood Units | OMICS International
ISSN: 2161-038X

Reproductive System & Sexual Disorders: Current Research
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Research Article

Application of a Novel High Yield Volume-Reduction Method for the Cryopreservation of 950 UC Blood Units

Nikos Tsagias1*, Kokkona Kouzi-Koliakou2 and George Koliakos3,4

1Department of Obstetrics and Gynecology, Democritus University of Thrace, Alexandroupolis, Greece

2Department of Histology-Embryology, Medical School, Aristotle University of Thessaloniki, 54124, Greece

3Department of Biological Chemistry, Medical School, Aristotle University Thessaloniki, Thessaloniki 54124, Greece

4Biohellenika SA, 65 Georgikis Scholis Avenue, 57001 Thermi, Thessaloniki, Greece

*Corresponding Author:
Nikos Tsagias
Department of Obstetrics and Gynecology
Democritus University of Thrace
Alexandroupolis, Greece
E-mail: [email protected]

Received date: July 22, 2013; Accepted date: August 19, 2013; Published date: August 26, 2013

Citation: Tsagias N, Kouzi-Koliakou K, Koliakos G (2013) Application of a Novel High Yield Volume-Reduction Method for the Cryopreservation of 950 UC Blood Units. Reprod Sys Sexual Disorders 2:123. doi:10.4172/2161-038X.1000123

Copyright: © 2013 Tsagias N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Umbilical cord blood represents an increasingly useful source of hematopoietic stem cells for Bone Marrow Transplantation (BMT), although currently, low cell numbers generally limit its use to pediatric patients. The aim of this study was to evaluate the routine use of a recently published volume reduction protocol for long term cord blood banking as was applied in a large number of cord blood samples. 950 cord blood samples with mean volume collected 78.81 ± 23.56 ml, Nucleated Cell (NC) 0.91 ± 0.44 × 109 NC, Monocytes (MN) 0.49 ± 0.23 × 109 MN and CD34+ HPCs (Haematopoietic Progenitors Stem Cells) 2.4 ± 2.08 × 106 CD34+ HPCs counts, have been collected respectively. We found a significant correlation between leukocyte counts and CD34+ HPCs with the volume collected (r=0.71, P<0.0001). We found a highly statistically significant correlation between leukocyte cell count and CD34+ HPCs cell number, (r=0.66, P<0.0001). After volume reduction the respective UCB units contained 1.06 ± 0.54 × 109 NC and 3.4 ± 3.07 × 106 CD34+ HPC , 17.88 ± 9.38 × 109 Red Blood Cells (RBC), while the viability and the volume were 92 ± 5.9 % and 6.43 ± 0.99 ml respectively.

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