alexa Application of a Novel High Yield Volume-Reduction Method for the Cryopreservation of 950 UC Blood Units | OMICS International | Abstract
ISSN: 2161-038X

Reproductive System & Sexual Disorders: Current Research
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Application of a Novel High Yield Volume-Reduction Method for the Cryopreservation of 950 UC Blood Units

Nikos Tsagias1*, Kokkona Kouzi-Koliakou2 and George Koliakos3,4

1Department of Obstetrics and Gynecology, Democritus University of Thrace, Alexandroupolis, Greece

2Department of Histology-Embryology, Medical School, Aristotle University of Thessaloniki, 54124, Greece

3Department of Biological Chemistry, Medical School, Aristotle University Thessaloniki, Thessaloniki 54124, Greece

4Biohellenika SA, 65 Georgikis Scholis Avenue, 57001 Thermi, Thessaloniki, Greece

*Corresponding Author:
Nikos Tsagias
Department of Obstetrics and Gynecology
Democritus University of Thrace
Alexandroupolis, Greece
E-mail: [email protected]

Received date: July 22, 2013; Accepted date: August 19, 2013; Published date: August 26, 2013

Citation: Tsagias N, Kouzi-Koliakou K, Koliakos G (2013) Application of a Novel High Yield Volume-Reduction Method for the Cryopreservation of 950 UC Blood Units. Reprod Sys Sexual Disorders 2:123. doi:10.4172/2161-038X.1000123

Copyright: © 2013 Tsagias N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Umbilical cord blood represents an increasingly useful source of hematopoietic stem cells for Bone Marrow Transplantation (BMT), although currently, low cell numbers generally limit its use to pediatric patients. The aim of this study was to evaluate the routine use of a recently published volume reduction protocol for long term cord blood banking as was applied in a large number of cord blood samples. 950 cord blood samples with mean volume collected 78.81 ± 23.56 ml, Nucleated Cell (NC) 0.91 ± 0.44 × 109 NC, Monocytes (MN) 0.49 ± 0.23 × 109 MN and CD34+ HPCs (Haematopoietic Progenitors Stem Cells) 2.4 ± 2.08 × 106 CD34+ HPCs counts, have been collected respectively. We found a significant correlation between leukocyte counts and CD34+ HPCs with the volume collected (r=0.71, P<0.0001). We found a highly statistically significant correlation between leukocyte cell count and CD34+ HPCs cell number, (r=0.66, P<0.0001). After volume reduction the respective UCB units contained 1.06 ± 0.54 × 109 NC and 3.4 ± 3.07 × 106 CD34+ HPC , 17.88 ± 9.38 × 109 Red Blood Cells (RBC), while the viability and the volume were 92 ± 5.9 % and 6.43 ± 0.99 ml respectively.

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A


[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


porn sex

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

Gaziantep Escort

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A


[email protected]

1-702-714-7001Extn: 9037


James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

mp3 indir

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals


Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T


[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7