Application of Analyte Harvesting Nanoparticle Technology to the Measurement of Urinary HGH in Healthy Individuals
2Pharmacology Research Unit, Municipal Institute of Medical Research (IMIM), Department of Experimental and Health Sciences, Pompeu Fabra University (UPF), C/ Doctor Aiguader 80, 08003-Barcelona, Spain
- *Corresponding Author:
- Alessandra Luchini
Center for Applied Proteomics and Molecular Medicine
George Mason University
10900 University Blvd BRH rm 325
Manassas VA 20110, USA
Tel: 703 993 8945
Fax: 703 993 8606
E-mail: [email protected]
Received Date: October 02, 2012; Accepted Date: October 26, 2012; Published Date: October 29, 2012
Citation: Luchini A, Tamburro D, Magni R, Fredolini C, Espina V, et al. (2012) Application of Analyte Harvesting Nanoparticle Technology to the Measurement of Urinary HGH in Healthy Individuals. J Sports Med Doping Stud 2:116. doi:10.4172/2161-0673.1000116
Copyright: © 2012 Luchini A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Urine represents a valuable biofluid for noninvasive measurement of Human Growth Hormone (HGH) secretion. Unfortunately, currently available commercial HGH immunoassays do not achieve the sensitivity needed for urinary HGH measurement in the low picogram per milliliter range, the expected normal concentration range of HGH in urine.
A nanotechnology based sample preprocessing step was used to extract and concentrate HGH in urine so that urinary HGH could be measured with a clinical grade standard immunoassay designed for serum (Immulite 1000 ,Siemens). We applied the nanoparticle enhanced immunoassay to evaluate the baseline value of urinary HGH in a population of healthy young adults (age 18-30, N=33, median 21, M: F=39%:61%, with no reported medical therapies).
Nanoparticle sample preprocessing effectively improved the lower limit of detection of the Immulite HGH assay by more than 50 fold, shifting the linear range of the assay to encompass the expected value of urinary HGH. The full process between run and within run CV% was 7.9 and 9.0%, respectively. On 33 healthy volunteers, the 95% reference values for hGH in spot urine normalized to specific gravity were 0.64 - 16.85 pg/mL (0.05 -5.82 ng/g creatinine).
Nanoparticle preprocessing constitutes a reliable means of measuring urinary HGH with a clinical grade
immunoassay, now establishing a normal baseline value for HGH in urine. Nanoparticles can be used to study the kinetics of HGH excretion in urine, and the factors that influence urinary HGH secretion and HGH isoform proportions.