Application of Fused-Core Particle Column in Two Dimensional Reversed Phase - Reversed Phase LC/MS Analysis of Biological Samples. Impact of Extra-Column Volume
Eduard Rogatsky*, Shaynah Browne, Bing Na Zheng and Daniel T. Stein
Biomarker Analytical Resource Core, Einstein-Montefiore Institute for Clinical and Translational Research, Albert Einstein College of Medicine, Bronx, NY 10461, USA
- *Corresponding Author:
- Eduard Rogatsky
Biomarker Analytical Resource Core
Einstein-Montefiore Institute for Clinical
and Translational Research
Albert Einstein College of Medicine
Bronx, NY 10461, USA
Received date: December 03, 2012; Accepted date: December 20, 2012; Published date: December 24, 2012
Citation: Rogatsky E, Browne S, Zheng BN, Stein DT (2012) Application of Fused- Core Particle Column in Two Dimensional Reversed Phase - Reversed Phase LC/ MS Analysis of Biological Samples. Impact of Extra-Column Volume. J Chromat Separation Techniq 3:158. doi:10.4172/2157-7064.1000158
Copyright: © 2012 Rogatsky E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
We demonstrate successful applicability of fused–core column in analysis of biological samples (diluted urine and SPE–pre-purified plasma or serum) using 2 dimensional chromatography. Implementation of fused-core material in the second dimension of 2D chromatographic platform allowed improving S/N above 4 fold in comparison to traditional porous C18 column. Sample load onto the first dimension (5 μm) eliminates limitations of sample volume to be injected. The main limitation factor of the fused-core material–low loading capacity was overcome by using 2D chromatography. We emphasize importance of reduction of post-column (extra column) volume in LC/MS applications utilizing fused-core columns.