alexa Application of Home-Made Enzyme and Biosurfactant in the Anaerobic Treatment of Effluent with High Fat Content | OMICS International | Abstract
ISSN: 2155-9821

Journal of Bioprocessing & Biotechniques
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Research Article

Application of Home-Made Enzyme and Biosurfactant in the Anaerobic Treatment of Effluent with High Fat Content

Jaqueline do Nascimento Silva1*, Melissa Limoeiro Estrada Gutarra1, Denise Maria Guimaraes Freire2 and Magali Christe Cammarota1
1Department of Biochemical Engineering, School of Chemistry, Universidade Federal do Rio de Janeiro, Federal University of Rio de Janeiro, Cidade Universitária,Centro de Tecnologia, Bl. E, Sl. 203, Ilha do Fundão, 21941-909, Rio de Janeiro, Brasil.
2Department of Biochemistry, Institute of Chemistry, Universidade Federal do Rio de Janeiro, Federal University of Rio de Janeiro, Cidade Universitária, Centro de Tecnologia, Bl. A, Sl. 549, Ilha do Fundão, 21949-900, Rio de Janeiro, Brasil.
Corresponding Author : Jaqueline do Nascimento
Department of Biochemical Engineering
School of Chemistry, Federal University of Rio de Janeiro
Cidade Universitária, Centro de Tecnologia
Bl. E, Sl. 203, Ilha do Fundão
21941-909, Rio de Janeiro, Brasil
Tel: 55-21-2562-7568
Fax: 55-21-2562-7567
E mail: [email protected]
Received October 18, 2013; Accepted November 21, 2013; Published November 27, 2013
Citation: Silva JdoN, Gutarra MLE, Freire DMG, Cammarota MC (2013) Application of Home-Made Enzyme and Biosurfactant in the Anaerobic Treatment of Effluent with High Fat Content. J Bioprocess Biotech 3:139 doi: 10.4172/2155-9821.1000139
Copyright: © 2013 Silva JdoN, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The SSF lipase production from Penicillium simplicissimum using an agro industrial residue and the biosurfactant from Pseudomonas aeruginosa were employed for a combined pre-treatment of wastewater from poultry processing industry. During the hydrolysis step of the wastewater fat better results were obtained at 34°C with biosurfactant 0.4% (v/v) and liquid enzyme preparation 6.2% (v/v) or at 46°C with biosurfactant 0.1% (v/v) and liquid enzyme preparation 3.8% (v/v). The pre-hydrolysis in two different conditions of enzyme and biosurfactant concentrations at 34°C and posterior anaerobic treatment allowed the COD removal and methane production, while the no hydrolyzed wastewater besides the COD removal, did not show methane production. Those results suggest the importance and efficiency of the pre-treatment with home-made lipases and biosurfactant during the high fat content wastewater pre-treatment.

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