alexa Applications of Rat Brain Synaptic Vesicle Proteins for Sensitive and Specific Detection of Botulinum Neurotoxins
ISSN: 2157-2526

Journal of Bioterrorism & Biodefense
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Applications of Rat Brain Synaptic Vesicle Proteins for Sensitive and Specific Detection of Botulinum Neurotoxins

Manglesh Kumar Singh, Vinita Chauhan and Ram Kumar Dhaked*

Biotechnology Division, Defence Research & Development Establishment, Gwalior-474002, India

*Corresponding Author:
Ram Kumar Dhaked
Biotechnology Division
Defence Research & Development Establishment
Gwalior-474002, MP, India
Tel: 91-751-2233489
Fax: 91-751-2341148
E-mail: [email protected]

Received Date: April 09, 2012; Accepted Date: May 16, 2012; Published Date: May 23, 2012

Citation: Singh MK, Chauhan V, Dhaked RK (2012) Applications of Rat Brain Synaptic Vesicle Proteins for Sensitive and Specific Detection of Botulinum Neurotoxins. J Bioterr Biodef S2:008. doi:10.4172/2157-2526.S2-008

Copyright: © 2012 Singh MK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



We propose here the application of synaptic vesicle proteins isolated from rat brain as a sole substrate for the specific endoproteinase activities of all seven serotypes of Botulinum Neurotoxin (BoNT/A to G). In this study, we used these proteins for evaluating endopeptidase and receptor binding activity for detecting BoNT/A by western blot and surface plasmon resonance with 6.25 pM and 0.22 fM limit of detection, respectively. Substrate and receptor present in the synaptic vesicle proteins are very robust and stable for more than 6 months to use in BoNT detection.


Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version