alexa Aqueous Extraction of Ergothioneine from Mycelia of Pleurotus ostreatus and Ergothioneine Accumulation Regularity during Submerged Fermentation
E- ISSN: 2320 - 3528
P- ISSN: 2347 - 2286

Research & Reviews: Journal of Microbiology and Biotechnology
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Research Article

Aqueous Extraction of Ergothioneine from Mycelia of Pleurotus ostreatus and Ergothioneine Accumulation Regularity during Submerged Fermentation

Weiya ZHANG1,2,3, Qi LIU1, Tao ZHOU1,2, Baoliang MEI1,2, Ning CHEN2, Wenxia JIANG1*

1Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, PR China

2College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300222, PR China

3Tianjin Century Pharmaceutical Co., Ltd, Tianjin 300160, PR China

*Corresponding Author:
Wenxia JIANG
Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
Tel: 86-22-84861941
E-mail: [email protected]

Received date: 08/09/2015 Accepted date: 08/01/2016 Published date: 01/02/2016

 

Abstract

Ergothioneine (EGT) can be produced by mycelia of mushrooms by submerged fermentation. In this study, we developed a brief method for extracting intracellular EGT. Hot water was selected as the appropriate solvent for its high safety and effectiveness during extraction process. With single-factor experiment and response surface methodology (RSM), the effects of the ratio of water to mycelia, temperature, stirring speed, processing time on the extract process were investigated and optimized. The results showed that on the conditions of the ratio of water to mycelia of 24.5: 1 (ml: g), temperature of 87.5°C, processing time of 10 min and stirring speed of 200 rpm, the extraction ratio of intracellular EGT is up to 97.1%, this method is simple and fast, thus it can be applied to industrialization conveniently. Moreover, this study showed that a small amount of extracellular EGT accumulation was observed during whole stage of fermentation, which is proposed to affect the intracellular EGT extraction and be useful for promoting EGT biosynthesis.

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