alexa Aqueous Two-Phase Extraction Advances for Bioseparation
ISSN: 2155-9821

Journal of Bioprocessing & Biotechniques
Open Access

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Review Article

Aqueous Two-Phase Extraction Advances for Bioseparation

Arafat M Goja1,2, Hong Yang1,3,4,5*, Min Cui6,7, Charles Li8
1College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China
2Department of Food Science and Technology, Faculty of Agriculture and Natural Resources, Bakht Alruda University, ED Dueim, 1311, Sudan
3Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, Wuhan, Hubei 430070, China
4National R&D Branch Center for Conventional Freshwater Fish Processing (Wuhan), Wuhan, Hubei 430070, China
5Aquatic Product Engineering and Technology Research Center of Hubei Province, Wuhan, Hubei 430070, China
6State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China
7Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China
8College of Food Science, Fujian Agricultural and Forestry University, Fuzhou, China
Corresponding Author : Hong Yang
College of Food Science and Technology
Huazhong Agricultural University, Wuhan, Hubei 430070, China
Tel: 86-27-87671045
Fax: 86-27-87278373
Email: [email protected]
Received November 07, 2013; Accepted November 25, 2013; Published December 05, 2013
Citation: Goja AM, Yang H, Cui M, Li C (2013) Aqueous Two-Phase Extraction Advances for Bioseparation. J Bioprocess Biotech 4:140. doi: 10.4172/2155-9821.1000140
Copyright: © 2013 Goja AM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

Aqueous two-phase extraction (ATPE), unique liquid-liquid extraction, involves a transfer of solute from one aqueous phase to another. ATPE includes polymer–polymer type and polymer–salt type systems for the recovery of proteins. The protein must be recovered in a highly purified form in order to improve its quality, decrease energy consumption, reduce waste and minimize costs. To acquire the high value and achieve good control over processes, the reliable, multi-component products are required especially those with the ability to investigate complex processing conditions. The current reviewing paper discusses the most recent progresses for the recovery of biomolecules by using the ATPE, covering the mechanism, which controls the phase formation and the behavior of solute partitioning in aqueous twophase systems (ATPS) processes. The review discusses also the increasing application for the recovery of high-value bioproducts, the recent development of alternative low cost ATPS and disadvantages attributed to ATPS.

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