alexa Array-Comparative-Genomic-Hybridization (Acgh) Based Preimplantation-Genetic-Diagnosis (PGD) for Balanced Translocation Carriers Improves both Diagnostic and Pregnancy Rates Compared to Fluorescent-In-Situ-Hybridization (FISH) and Polymerase-Chain-Reaction (PCR) Based PGD
ISSN: 2375-4508

Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biology
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Research Article

Array-Comparative-Genomic-Hybridization (Acgh) Based Preimplantation-Genetic-Diagnosis (PGD) for Balanced Translocation Carriers Improves both Diagnostic and Pregnancy Rates Compared to Fluorescent-In-Situ-Hybridization (FISH) and Polymerase-Chain-Reaction (PCR) Based PGD

Tali Mishael1,4, Talia Eldar-Geva1,3,4, Sharon Zeligson2,4, Rachel Beeri2, David A Zeevi2, Paul Renbaum2, Orit Lobel2, Yafa Nevo2, Raphael Ron- El4, Ehud J Margalioth1, Ephrat Levy-Lahad2,3 and Gheona Altarescu2,3*
1Reproductive Endocrinology and Genetics Unit, IVF Unit, Department of Obstetrics and Gynecology, Shaare Zedek Medical Center, Jerusalem, Israel
2Medical Genetics Institute, ‘ZOHAR’ PGD Lab, Shaare Zedek Medical Center, Jerusalem, Israel
3Hebrew University School of Medicine, Jerusalem, Israel
4IVF and Infertility Unit, Assaf Harofeh Medical Center, Sackler School of Medicine, Tel-Aviv University, Zerifin, Israel
*Corresponding Author : Gheona Altarescu
Medical Genetics Institute
‘ZOHAR’ PGD Lab
Shaare Zedek Medical Center
Jerusalem, Israel
Tel: 97226666435
Fax: 97226666935
E-mail: [email protected]
Received January 25, 2015; Accepted March 31, 2015; Published April 07, 2015
Citation: Mishael T, Eldar-Geva T, Zeligson S, Beeri R, Zeevi DA, et al. (2015) Array-Comparative-Genomic-Hybridization (Acgh) Based Preimplantation-Genetic- Diagnosis (PGD) for Balanced Translocation Carriers Improves both Diagnostic and Pregnancy Rates Compared to Fluorescent-In-Situ-Hybridization (FISH) and Polymerase-Chain-Reaction (PCR) Based PGD. J Fertil In Vitro IVF Worldw Reprod Med Genet Stem Cell Biol 3:147. doi:10.4172/2375-4508.1000147
Copyright: ©2015 Mishael T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Introduction: Preimplantation genetic diagnosis (PGD) of balanced translocations is usually performed using Fluorescence-in-Situ-Hybridization (FISH), and more rarely by Polymerase-Chain-Reaction (PCR). These methods assess only chromosomes involved in the translocation, as opposed to array comparative-genomic-hybridization (aCGH) which screens all chromosomes.

Material and Methods: In this report, we compared the diagnosis and pregnancy rates for balanced translocations using all three techniques in a retrospective study of 75 PGD cycles.

Results: Of 331 embryos analyzed by FISH, 225 (68%) were successfully diagnosed, 34 (15%) were transferable yielding a pregnancy rate of 13% per Ovum-Pick-Up (OPU). With PCR, 80 out of 124 embryos (65%) were diagnosed, 21 (26%) were transferable, and a pregnancy rate of 14.3% per OPU was achieved. Using aCGH, 60 of 71 embryos (85%) were successfully diagnosed, 26 of which (43%) presented with chromosomal aneuploidies not associated with the parental translocation. Nine embryos diagnosed by aCGH were transferable yielding a pregnancy rate of 40% per OPU. The proportion of embryos successfully diagnosed and pregnancy rates per OPU were significantly higher using aCGH compared to FISH or PCR (p= 0.036; p=0.017), respectively.

Conclusion: Altogether, these data indicate that the use of aCGH for chromosomal translocation PGD significantly increases diagnosis and pregnancy rates.

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