Array-Comparative-Genomic-Hybridization (Acgh) Based Preimplantation-Genetic-Diagnosis (PGD) for Balanced Translocation Carriers Improves both Diagnostic and Pregnancy Rates Compared to Fluorescent-In-Situ-Hybridization (FISH) and Polymerase-Chain-Reaction (PCR) Based PGD
|Tali Mishael1,4, Talia Eldar-Geva1,3,4, Sharon Zeligson2,4, Rachel Beeri2, David A Zeevi2, Paul Renbaum2, Orit Lobel2, Yafa Nevo2, Raphael Ron- El4, Ehud J Margalioth1, Ephrat Levy-Lahad2,3 and Gheona Altarescu2,3*|
|1Reproductive Endocrinology and Genetics Unit, IVF Unit, Department of Obstetrics and Gynecology, Shaare Zedek Medical Center, Jerusalem, Israel|
|2Medical Genetics Institute, ‘ZOHAR’ PGD Lab, Shaare Zedek Medical Center, Jerusalem, Israel|
|3Hebrew University School of Medicine, Jerusalem, Israel|
|4IVF and Infertility Unit, Assaf Harofeh Medical Center, Sackler School of Medicine, Tel-Aviv University, Zerifin, Israel|
|*Corresponding Author :||Gheona Altarescu
Medical Genetics Institute
‘ZOHAR’ PGD Lab
Shaare Zedek Medical Center
E-mail: [email protected]
|Received January 25, 2015; Accepted March 31, 2015; Published April 07, 2015|
|Citation: Mishael T, Eldar-Geva T, Zeligson S, Beeri R, Zeevi DA, et al. (2015) Array-Comparative-Genomic-Hybridization (Acgh) Based Preimplantation-Genetic- Diagnosis (PGD) for Balanced Translocation Carriers Improves both Diagnostic and Pregnancy Rates Compared to Fluorescent-In-Situ-Hybridization (FISH) and Polymerase-Chain-Reaction (PCR) Based PGD. J Fertil In Vitro IVF Worldw Reprod Med Genet Stem Cell Biol 3:147. doi:10.4172/2375-4508.1000147|
|Copyright: ©2015 Mishael T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
Introduction: Preimplantation genetic diagnosis (PGD) of balanced translocations is usually performed using Fluorescence-in-Situ-Hybridization (FISH), and more rarely by Polymerase-Chain-Reaction (PCR). These methods assess only chromosomes involved in the translocation, as opposed to array comparative-genomic-hybridization (aCGH) which screens all chromosomes.
Material and Methods: In this report, we compared the diagnosis and pregnancy rates for balanced translocations using all three techniques in a retrospective study of 75 PGD cycles.
Results: Of 331 embryos analyzed by FISH, 225 (68%) were successfully diagnosed, 34 (15%) were transferable yielding a pregnancy rate of 13% per Ovum-Pick-Up (OPU). With PCR, 80 out of 124 embryos (65%) were diagnosed, 21 (26%) were transferable, and a pregnancy rate of 14.3% per OPU was achieved. Using aCGH, 60 of 71 embryos (85%) were successfully diagnosed, 26 of which (43%) presented with chromosomal aneuploidies not associated with the parental translocation. Nine embryos diagnosed by aCGH were transferable yielding a pregnancy rate of 40% per OPU. The proportion of embryos successfully diagnosed and pregnancy rates per OPU were significantly higher using aCGH compared to FISH or PCR (p= 0.036; p=0.017), respectively.
Conclusion: Altogether, these data indicate that the use of aCGH for chromosomal translocation PGD significantly increases diagnosis and pregnancy rates.