Assess the Possibility of Screening and Supervising Influenza A (H7N9) Virus-Infected Patients via Rapid Point-of-Care-Test Kit with Upper or Lower Respiratory Specimens
1State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, National Clinical Centre of Respiratory Disease, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou, China
- *Corresponding Author:
- Zifeng Yang
State Key Laboratory of Respiratory Diseases
Guangzhou Institute of Respiratory Disease
National Clinical Centre of Respiratory Disease
The First Affiliated Hospital, Guangzhou Medical University
Tel: + 853 8897 2326
E-mail: [email protected]
Guangdong Entry-exit Inspection and Quarantine Bureau Technology Center
E-mail: [email protected]
Received date: September 14, 2016; Accepted date: October 03, 2016; Published date: October 07, 2016
Citation: Li Z, Li L, Wu S, Li X, Li R, et al. (2016) Assess the Possibility of Screening and Supervising Influenza A (H7N9) Virus-Infected Patients via Rapid Point-of-Care-Test Kit with Upper or Lower Respiratory Specimens. J Pulm Respir Med 6: 371. doi: 10.4172/2161-105X.1000371
Copyright: © 2016 Li Z, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The purpose of this study was to assess the possibility of screening and supervising influenza A (H7N9) virus infected patients via rapid point-of-care-test kit with upper or lower respiratory specimens. Here, we demonstrate that the POCT kits can detect H7N9 virus (Guangzhou strain) in different type of samples, which obtained from upper and lower respiratory tract, via the simulated experiment. The detection limit ranged from 4 to 6 log10TCID50/mL and 2.78 × 107 to 1.05 × 108 copies/mL in PBS and nasopharyngeal swab solution (NSS) solutions respectively. Furthermore, the detection ranges were enhanced by 0.5 or 1 log10TCID50/mL in BALF or sputum solution (SS) diluents for some parts of the POCT kits. Furthermore, we observed that there were higher replication and longer screening of H7N9 virus in lower respiratory tract (LRT) samples, such as endotracheal aspirate (ETA) and sputum, from the H7N9 virus-infected patients, although the detection results of POCT kits were negative for these clinical samples. So that, we think that POCT kits can be used to screen and supervise H7N9 virus-infected patients if in the accurate timing. And the LRT samples, special ETA and sputum, seem a good sample option for detecting H7N9 virus via POCT kits, so we need pay more attention to the LRT samples when the new POCT kits were developed.