Assessing the Gametocyte Production in Field Isolates of Plasmodium falciparumPurva Gupta, Veena Yadavendu and Vineeta Singh*
National Institute of Malaria Research (ICMR), Sector-8 Dwarka, New Delhi, India
- *Corresponding Author:
- Vineeta Singh
National Institute of Malaria Research (ICMR)
Sector-8 Dwarka, New Delhi, India
Email: [email protected]
Received date: October 05, 2015; Accepted date: November 18, 2015; Published date: November 23, 2015
Citation: Gupta P, Yadavendu V, Singh V (2015) Assessing the Gametocyte Production in Field Isolates of Plasmodium falciparum. J Bacteriol Parasitol 6:250. doi: 10.4172/2155-9597.1000250
Copyright: © 2015 Singh V, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The production of gametocytes in field isolates of Plasmodium falciparum is not clearly understood even though the gametocytes are crucial for disease transmission. Samples collected during the malaria transmission period from two different regions of India were cultured in vitro for gametocyte production and analyzed by PCR and RT-PCR assay for Pfs25 gene. A total of 20 P. falciparum field isolates were collected which showed varying intensity of in vitro gametocyte production. The isolates which produced mature gametocytes in vitro also had an increase in their Pfs25 expression, indicating that the gametocyte produced is directly proportional to the Pfs25 gene expression. The expression ranged from 0.32 to 4.56 fold in field isolates when compared to the reference strain. The in vitro gametocyte production in fresh field isolates directly correlated with the expression of Pfs25 gene in these isolates as shown by ANOVA test.