Assessment of Cytogenetic Instability and Gene Transcription of Chronic Myelogenous Leukemia Cells Exposed to Non-thermal Plasma
1`Department of Biological Application, Nuclear Research Center, Atomic Energy Authority, Cairo, Egypt
- *Corresponding Author:
- Ahmed MM
Department of Radiation Biology
National Center for Radiation Research and Technology (NCRRT)
Nasr city, Egypt
Tel: 00202 6713144
E- mail: [email protected]
Received Date: August 02, 2015; Accepted Date: September 04, 2015; Published Date: September 06, 2015.
Citation: Elhadary AMA, El-Aragi GM, Ahmed MM, Said ZS (2015) Assessment of Cytogenetic Instability and Gene Transcription of Chronic Myelogenous Leukemia Cells Exposed to Non-thermal Plasma. J Cytol Histol 6:371. doi:10.4172/2157-7099.1000371
Copyright: © 2015 Elhadary AAM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: This study investigates the effect of Atmospheric Pressure Plasma Jet (APPJ) on cytogenetic instability and transcription of genes associated with propagation of chronic myelogenous leukemia (CML).
Material and methods: Blood samples were collected from six patients with CML and compared with control group. The first three cases were in accelerated phase (AP-CML) resistant to imatinib and nilotinib drugs. The others partially responded and returned to chronic phase (CP-CML). Triple blood cultures for each case were exposed to single dose of plasma, with treatment times of 40, 80, and 120 sec. Mitotic phases, indices and abnormalities, as well transcription levels of P53, caspase-3 and BcL-2 genes were detected before and after plasma jet exposure.
Results and conclusion: The data showed that the exposure to APPJ caused remission of the mitotic indices and give a significant increase in the arresting of chromosome instabilities by induction of different mitotic checkpoints and express of sticky and condensed chromosomes which ended by apoptosis and necrosis processes. Additionally, the same events detected for the transcriptional levels of P53, caspase-3 and Bcl-2 genes.