Assessment of Genetic Variations among Medicinal Plant Cassia tora from Different Geographic Regions of Central India Using RAPD MarkersAnita Tilwari*, Deepak Chauhan, Rajesh Sharma and RK Singh
Centre of Excellence in Biotechnology, MP Council of Science and Technology, Vigyan Bhawan, Nehru Nagar, Bhopal, Madhya Pradesh, India
- *Corresponding Author:
- Anita Tilwari
Centre of Excellence in Biotechnology
MP Council of Science and Technology, Vigyan Bhawan
Nehru Nagar, Bhopal, Madhya Pradesh, India
E-mail: [email protected]
or [email protected]
Received date: November 02, 2016; Accepted date: November 15, 2016; Published date: November 24, 2016
Citation: Tilwari A, Chauhan D, Sharma R, Singh RK (2016) Assessment of Genetic Variations among Medicinal Plant Cassia tora from Different Geographic Regions of Central India Using RAPD Markers. Med Aromat Plants (Los Angel) 5:276. doi: 10.4172/2167-0412.1000276
Copyright: © 2016 Tilwari A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In this study 13 different accessions of medicinal and aromatic plant Cassia tora obtained from different agro climatic zones of Madhya Pradesh (Central India) were evaluated for its genetic variability using the random amplified polymorphic DNA (RAPD) markers. During RAPD analysis, amplification of genomic DNA of the 13 accessions by 10 selected primers yielded 88 bright and discernible bands, of which 86 were polymorphic with an average of 9.6 bands per primer and polymorphism obtained was 97.72%. The amplified products varied in size from 2,111 to 153 bp. The Jaccard similarity coefficient value ranged from 0.23 to 0.65 indicate that the distribution of variation is diverse. A dendrogram constructed based on the UPGMA clustering method revealed three major clusters, which are again divided into sub clusters. The result revealed rich genetic diversity among Cassia tora accessions obtained from different agro climatic zones of central India, which gives its suitability to grow and conserve anywhere at any environmental conditions.